Disseminated Mycobacterium avium complex infection in an immunocompetent pregnant woman
© Song et al; licensee BioMed Central Ltd. 2006
Received: 19 April 2006
Accepted: 22 October 2006
Published: 22 October 2006
Disseminated mycobacterium avium complex (MAC) occurs mainly in immunocompromised hosts, which is associated with abnormal cellular immunity.
A 26-year-old pregnant woman presented with fever and general weakness. Miliary lung nodules were noted on chest X-ray. Under the impression of miliary tuberculosis, anti-tuberculosis medication was administered. However, the patient was not improved. Further work-up demonstrated MAC in the sputum and placenta. The patient was treated successfully with clarithromycin-based combination regimen.
This appears to be the first case of disseminated MAC in an otherwise healthy pregnant woman. Clinicians should be alert for the diagnosis of MAC infection in diverse clinical conditions.
MAC has been found to colonize natural water, soil, indoor water systems, and hot tubs, and it can be isolated from the sputum of apparently healthy individuals [3, 4]. Clinically, MAC pulmonary disease is more frequently seen in patients with underlying lung disease such as bronchiectasis, pneumoconiosis, and prior tuberclulosis, but approximately 24 to 59 percent of them occur in immunocompetent persons without evident predisposing factors [2, 5].
In comparison, disseminated MAC disease has been mainly noted in immunocompromised hosts with impaired cellular immunity . Recently, we experienced an interesting case of disseminated MAC disease, which occurred in an otherwise healthy pregnant woman. Pregnancy might affect on the patient's immune status. Herein, we report the unusual case with the review of previously published literatures.
In ROK, tuberculosis is still highly prevalent, so the patient was initially suspected of having miliary tuberculosis in the lung. Anti-tuberculosis medication was started with isoniazid (300 mg qd), rifampin (600 mg qd), ethambutol (1.2 g qd), and pyrazinamide (1.5 g qd). Aminotransferase levels were not elevated further during the course of anti-tuberculosis medication. Sputum induction with hypertonic saline was performed because the patient only had intermittent dry cough and scanty sputum. The sputum study was negative for Mycobacterium on Ziehl-Neelson stain.
High fever persisted despite anti-tuberculosis medication. On day 15 of anti-tuberculosis medication, the patient's condition was aggravated; she suffered from severe dyspnea and hyperemesis. Fetal distress was suspected, forcing us to terminate the pregnancy. Pathologic specimens obtained from the placenta demonstrated many acid fast bacilli in Ziehl-Neelson stain (Fig. 1B). However, the patient was still febrile despite the termination of pregnancy and continued anti-tuberculosis medication. The authors believed that an immunologic response might have been responsible for the high fever and added oral steroid (oral prednisone; 1 mg/kg) to the patient's medication. The patient felt better and was no longer febrile. The patient was subsequently discharged from the hospital with those medications.
During outpatient follow-up, the patient complained of frequent episodes of fever and exertional dyspnea even though she was taking anti-tuberculosis medication. Chest X-ray revealed that the pulmonary disease worsened with multiple nodules in the entire lung. On day 45 of anti-tuberculosis medication, results from the initial series of sputum mycobacterial cultures on ogawa medium were available; Mycobacterium other than tuberculosis (MOTT) was demonstrated repeatedly. Result from placenta was same also. The patient's chest X-ray showed no improvement since discharge from the hospital, with persistent multiple tiny nodules in both lung fields. The medication was changed to clarithromycin (500 mg bid), rifampin (600 mg qd), ofloxacin (300 mg bid), and ethambutol (1.2 g qd).
We report a rare disseminated MAC case in an otherwise healthy, pregnant woman; there had been only sporadic case reports of disseminated MAC disease among immunocompetent adults [6–10]. Though the patient in this case was a HBV carrier, there was no evidence of liver cirrhosis on physical examination and abdominal sonogram. Platelet count was also normal. Repeated HIV serology was negative and tuberculin test showed strong positive reflecting normal cell-mediated immunity. Studies of malignancy work-up showed no abnormal finding.
Disseminated MAC disease is typically associated with defective cellular immunity such as hairy cell leukemia, chronic myelogenous leukemia and AIDS . Tumor necrosis factor (TNF)-α and IFN-γ are both important in the defense against mycobacterial disease. Schematically, stimulated CD4+ T-lymphocytes produce IL-12 and IFN-γ. These cytokines act on lymphocytes and macrophages to stimulate the production of IL-2, TNF-α, and reactive oxidative intermediates such as superoxide and nitric oxide that participate in the host defense against mycobacteria . Patients with lymphoreticular malignancies or AIDS are at risk of disseminated MAC infection, usually when the CD4+ count is <50 μL. In those patients, macrophages are unable to kill the intracellular mycobacteria largely because they lack the help of CD4+ T-cells secreting INF-γ and TNF-α; these cytokines are necessary for the activation of macrophages.
Alterations of the immune status in the pregnant woman are necessary to allow mothers to tolerate genetically different fetal tissues during pregnancy. These alterations lead to impaired cell-mediated immunity with increased susceptibility to certain infections such as tuberculosis [13, 14]. During pregnancy, the maternal immune system also shows a relative bias toward T helper (type 2) immunity. Therefore, we presumed that disseminated MAC infection in this case might be related to pregnancy state, even though immune-suppressed disease did not exist. Likewise, cytomegalovirus infection and disseminated aspergillosis had been reported as opportunistic infections in the pregnant women [15, 16].
Since the mid 1990s, genetic abnormalities are known to be predisposed to infection by intracellular pathogens including MAC and other mycobacteria; mutations in the interferon γ receptor 1 (IFN-γR1) gene and natural resistance-associated macrophage protein (nramp) gene have been reported . This case however, was not analyzed for these genes because no other family member revealed mycobacterial disease.
As for the diagnosis of disseminated MAC infection, though blood culture was not taken, positive cultures were identified from more than one organ (lung and placenta), and the patient showed compatible clinical/laboratory findings. Multi-drug therapy including clarithromycin or azithromycin is recommended for the treatment of disseminated MAC patients with AIDS based on available data [17, 18]. In contrast, no standard treatment has been established for HIV-negative patients with disseminated MAC infection. In this case, we treated the patient with combination of rifampin, ofloxacin and clarithromycin, and it was effective. We excluded ethambutol from the treatment regimen according to the in vitro susceptibility test. Actually, however, the susceptibility test of MAC has no value in guiding the selection of drugs other than clarithromycin .
In this study, CD4 T cell count was not checked during follow-up period, which was the limitation of this report. However, the patient was previously healthy and WBC count was within normal range. The positive conversion of tuberculin test was checked during treatment period after therapeutic abortion, therefore we thought those could be an indirect evidence of intact cellular immunity.
In conclusion, this case highlights a series of problems associated with MAC disease including delay in diagnosis, undefined mode of transmission, potential severity of the disease, and difficulty in treatment. Clinicians should be alert for the diagnosis of MAC infection in diverse clinical conditions. Large-scale study is needed in those patients with disseminated MAC disease, who are immunocompetent and free from underlying lung disease: treatment regimens, treatment duration, virulence factors of MAC, and host immunity.
We report a rare disseminated MAC case in an otherwise healthy, pregnant woman. The patient was treated successfully with combination of rifampin, ofloxacin and clarithromycin. Clinicians should be alert for the diagnosis of MAC infection in diverse clinical conditions.
Written consent was obtained from the patient for the publication of this case report.
- O'Brien RJ: The epidemiology of nontuberculous mycobacterial disease. Clin Chest Med. 1989, 10 (3): 407-418.PubMedGoogle Scholar
- Prince DS, Peterson DD, Steiner RM, Gottlieb JE, Scott R, Israel HL, Figueroa WG, Fish JE: Infection with Mycobacterium avium complex in patients without predisposing conditions. N Engl J Med. 1989, 321 (13): 863-868.View ArticlePubMedGoogle Scholar
- Tobin-D'Angelo MJ, Blass MA, del Rio C, Halvosa JS, Blumberg HM, Horsburgh CRJ: Hospital water as a source of Mycobacterium avium complex isolates in respiratory specimens. J Infect Dis. 2004, 189 (1): 98-104.View ArticlePubMedGoogle Scholar
- Horsburgh CRJ, Chin DP, Yajko DM, Hopewell PC, Nassos PS, Elkin EP, Hadley WK, Stone EN, Simon EM, Gonzalez P, et al: Environmental risk factors for acquisition of Mycobacterium avium complex in persons with human immunodeficiency virus infection. J Infect Dis. 1994, 170 (2): 362-367.View ArticlePubMedGoogle Scholar
- Huang JH, Oefner PJ, Adi V, Ratnam K, Ruoss SJ, Trako E, Kao PN: Analyses of the NRAMP1 and IFN-gammaR1 genes in women with Mycobacterium avium-intracellulare pulmonary disease. Am J Respir Crit Care Med. 1998, 157 (2): 377-381.View ArticlePubMedGoogle Scholar
- King BF: Disseminated Mycobacterium avium complex in an immunocompetent previously healthy woman. J Am Board Fam Pract. 1994, 7 (2): 145-148.PubMedGoogle Scholar
- Kwong JS, Munk PL, Connell DG, Gianoulis ME: Case report 687. Disseminated Mycobacterium avium-intracellulare osteomyelitis. Skeletal Radiol. 1991, 20 (6): 458-462. 10.1007/BF00191092.View ArticlePubMedGoogle Scholar
- Lai CC, Lee LN, Ding LW, Yu CJ, Hsueh PR, Yang PC: Emergence of disseminated infections due to nontuberculous mycobacteria in non-HIV-infected patients, including immunocompetent and immunocompromised patients in a university hospital in Taiwan. J Infect. 2006, 53 (2): 77-84.View ArticlePubMedGoogle Scholar
- Myojo M, Fujiuchi S, Matsumoto H, Yamazaki Y, Takahashi M, Satoh K, Takeda A, Nishigaki Y, Okamoto K, Fujita Y, Fujikane T, Shimizu T: Disseminated Mycobacterium avium complex (DMAC) in an immunocompetent adult. Int J Tuberc Lung Dis. 2003, 7 (5): 498-501.PubMedGoogle Scholar
- Greinert U, Rusch-Gerdes S, Vollmer E, Schlaak M: Extensive mediastinal lymphadenopathy in an adult immunocompetent woman caused by Mycobacterium avium complex. Chest. 1999, 116 (6): 1814-1816. 10.1378/chest.116.6.1814.View ArticlePubMedGoogle Scholar
- Horsburgh CRJ, Mason UG, Farhi DC, Iseman MD: Disseminated infection with Mycobacterium avium-intracellulare. A report of 13 cases and a review of the literature. Medicine (Baltimore). 1985, 64 (1): 36-48.View ArticleGoogle Scholar
- Hartmann P, Plum G: Immunological defense mechanisms in tuberculosis and MAC-infection. Diagn Microbiol Infect Dis. 1999, 34 (2): 147-152. 10.1016/S0732-8893(99)00005-X.View ArticlePubMedGoogle Scholar
- Szekeres-Bartho J, Wegmann TG: A progesterone-dependent immunomodulatory protein alters the Th1/Th2 balance. J Reprod Immunol. 1996, 31 (1-2): 81-95. 10.1016/0165-0378(96)00964-3.View ArticlePubMedGoogle Scholar
- Yip L, McCluskey J, Sinclair R: Immunological aspects of pregnancy. Clin Dermatol. 2006, 24 (2): 84-87. 10.1016/j.clindermatol.2005.10.022.View ArticlePubMedGoogle Scholar
- Biedermann K, Flepp M, Fierz W, Joller-Jemelka H, Kleihues P: Pregnancy, immunosuppression and reactivation of latent toxoplasmosis. J Perinat Med. 1995, 23 (3): 191-203.View ArticlePubMedGoogle Scholar
- Klock C, Cerski M, Dargel A, Goldani LZ: Case Report. Disseminated aspergillosis complicating pregnancy. Mycoses. 2002, 45 (9-10): 408-410. 10.1046/j.1439-0507.2002.00771.x.View ArticlePubMedGoogle Scholar
- Chaisson RE, Benson CA, Dube MP, Heifets LB, Korvick JA, Elkin S, Smith T, Craft JC, Sattler FR: Clarithromycin therapy for bacteremic Mycobacterium avium complex disease. A randomized, double-blind, dose-ranging study in patients with AIDS. AIDS Clinical Trials Group Protocol 157 Study Team. Ann Intern Med. 1994, 121 (12): 905-911.View ArticlePubMedGoogle Scholar
- Griffith DE, Brown BA, Girard WM, Griffith BE, Couch LA, Wallace Jr RJ: Azithromycin-containing regimens for treatment of Mycobacterium avium complex lung disease. Clin Infect Dis. 2001, 32 (11): 1547-1553. 10.1086/320512.View ArticlePubMedGoogle Scholar
- Diagnosis and treatment of disease caused by nontuberculous mycobacteria. This official statement of the American Thoracic Society was approved by the Board of Directors, March 1997. Medical Section of the American Lung Association. Am J Respir Crit Care Med. 1997, 156 (2 Pt 2): S1-25.
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/6/154/prepub
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