Fungemia due to Lachancea fermentati: a case report
© Leuck et al.; licensee BioMed Central Ltd. 2014
Received: 10 January 2014
Accepted: 1 May 2014
Published: 10 May 2014
Lachancea fermentati is an environmental yeast that is also used in the fermentation of alcoholic drinks. It has not previously been described as a human pathogen although the closely related yeast, Saccharomyces boulardii, can cause fungemia. Here we report a case of L. fermentati acting as a pathogen in a septic patient with cultures positive from blood, peritoneal fluid, bile, and sputum.
A 36 year-old Caucasian man was hospitalized with acute alcoholic hepatitis complicated by Escherichia coli spontaneous bacterial peritonitis. Three days after admission, he developed new fevers with sepsis requiring mechanical ventilation and vasopressor support. He was found to have a bowel perforation. Cultures from blood, peritoneal fluid, and sputum grew a difficult-to-identify yeast. Micafungin was started empirically. On hospital day 43 the yeast was identified as L. fermentati with low minimum inhibitory concentrations (by Epsilometer test) to all antifungals tested. Micafungin was changed to fluconazole to complete a 3-month course of therapy. Serial peritoneal fluid cultures remained positive for 31 days. One year after his initial hospitalization the patient had ongoing cirrhosis but had recovered from fungemia.
This case demonstrates the need for clinicians to consider host factors when interpreting culture results with normally non-pathogenic organisms. In this immunocompromised host L. fermentati caused disseminated disease. We believe his hobby of brewing alcohol led to colonization with L. fermentati, which then resulted in invasive disease when the opportunity arose.
KeywordsLachancea fermentati Fungemia Opportunistic pathogen
Non-Candida fungemia has become increasingly problematic among immunocompromised hosts, and fungal genera such as Trichosporon, Cryptococcus, Rhodotorula, Malassezia, and Blastoschizomyces have become recognized as opportunistic pathogens [1–3]. The genus Saccharomyces has also been described as a human pathogen [4–6]. We describe a case of fungemia and sepsis due to the yeast Lachancea fermentati, a species closely related to the saccharomycetes. The known potential of Saccharomyces to act as a pathogen suggests that the related species, L. fermentati, may also have the ability to cause disease in the appropriate clinical setting.
Summary of positive cultures during hospitalization
Hospital day of culturea
Site of isolation
L. fermentati, Enterococcus spp., Lactobacillus
L. fermentati, Candida rugosa
L. fermentati, coagulase negative staphylococcus
L. fermentati, Candida parapsilosis, Bacteroides spp., Pseudomonas aeruginosa, Clostridium spp.
C. parapsilosis, coagulase negative staphylococcus
C. parapsilosis, coagulase negative staphylococcus
Lachancea fermentati minimum inhibitory concentrations (MIC) by Epsilometer test (E-test)
MIC by E-test (ug/mL)
L. fermentati is an environmental, saprophytic yeast found in decaying material. In its natural setting, Lachancea colonizes leaf surfaces and may provide a natural buffer against plant pathogens . Lachancea species, in combination with Saccharomyces cerevisiae, are commonly used in the fermentation process to make wine and cachaca (a drink made from fermented sugar) [8, 9], and have been shown to enhance the quality and aroma of these beverages .
While L. fermentati has been recognized as a component of fermented drinks, it may be more pervasive than previously thought. L. fermentati was found in more than 50% of olive oil mills tested , and an investigation of commercially available drinks in Brazil also found L. fermentati in coconut juice and reconstituted fruit juices . A species with a close evolutionary relationship to L. fermentati, L. thermotolerans, is present on the leaves of deciduous trees in the autumn when fruit would be harvested . With this increasing recognition of Lachancea’s environmental presence, clinicians and clinical microbiologists should be aware of its pathogenic potential.
Recent progress has also been made in the classification of Lachancea yeasts. L. fermentati, formerly known as Zygosaccharomyces fermentati, is related to the genus Saccharomyces. Prior to DNA sequencing, classification of these organisms was based on morphologic and phenotypic characteristics. The advent of nuclear-based rDNA sequencing technology, coupled with multigene-based phylogenetic analyses, has led to reclassification of the 11 clades of Saccharomycetaceae. The genus Lachancea consists of five species, L. cidri, L. fermentati, L. kluyveri, L. thermotolerans and L. waltii. The organism is further characterized by vegetative reproduction with multilateral budding on a narrow base and fermentation of glucose in addition to at least one other sugar . Based on comparative analyses of rDNA sequences and molecular karyotyping of Lachancea species, the yeast appears to have eight chromosomes , and its preferred growing temperature is 25-37°C . This range includes normal human body temperature and suggests that L. fermentati could act as a pathogen.
Of interest, one of this patient’s early paracenteses grew Eggerthella lenta, which is associated with the gastrointestinal flora of wine drinkers . This patient did indeed brew his own alcohol. Taken together, the presence of two microorganisms associated with fermented drinks suggests that his microbiome may have been affected by his alcohol brewing and consumption, resulting in infection with an unusual pathogen, L. fermentati. In a similar case, S. cerevisiae (baker’s yeast) caused disseminated disease in a baker’s wife with leukemia, which was thought to be associated with occupation-related colonization .
To the best of our knowledge, this is the first case reporting L. fermentati as a human pathogen. This case of L. fermentati fungemia highlights the ability of non-pathogenic microorganisms to cause disease in unusual situations depending on host risk factors and clinical circumstances. If this patient had not been fungemic and septic, this yeast might have been considered a colonizing species. However, in this immunocompromised host with a perforated viscus, it acted as a pathogen and required treatment.
Written informed consent was obtained from the patient for publication of this case report. A copy of the written consent is available for review by the Editor of this journal.
We would like to thank Annette Fothergill at the Fungus Testing Laboratory at the University of Texas Health Science Center for identification of the yeast species. AML is supported by a National Institutes of Health Ruth L. Kirschstein National Research Service Award Institutional Research Training Grant [5T32AI055433-09]. JG and MR do not have grant support.
- Yamamoto M, Takakura S, Hotta G, Matsumura Y, Matsushima A, Nagao M, Ito Y, Ichiyama S: Clinical characteristics and risk factors of non-Candidafungaemia. BMC Infect Dis. 2013, 13: 247-10.1186/1471-2334-13-247.View ArticlePubMedPubMed CentralGoogle Scholar
- Colombo AL, Padovan AC, Chaves GM: Current knowledge o Trichosporonspp. and Trichosporonosis. Clin Microbiol Rev. 2011, 24 (4): 682-700. 10.1128/CMR.00003-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Gaitanis G, Magiatis P, Hantschke M, Bassukas ID, Velegraki A: The Malassezia genus in skin and systemic diseases. Clin Microbiol Rev. 2012, 25 (1): 106-141. 10.1128/CMR.00021-11.View ArticlePubMedPubMed CentralGoogle Scholar
- Thygesen JB, Glerup H, Tarp B: Saccharomyces boulardiifungemia caused by treatment with a probioticum. BMJ Case Rep. 2012, doi:10.1136/bcr.06.2011.4412,Google Scholar
- Hamoud S, Keidar Z, Hayek T: Recurrent Saccharomyces cerevisiaefungemia in an otherwise healthy patient. Isr Med Assoc J. 2011, 13 (9): 575-576.PubMedGoogle Scholar
- Cassone M, Serra P, Mondello F, Girolamo A, Scafetti S, Pistella E, Venditti M: Outbreak of Saccharomyces cerevisiaesubtype boulardii fungemia in patients neighboring those treated with a probiotic preparation of the organism. J Clin Microbiol. 2003, 41 (11): 5340-5343. 10.1128/JCM.41.11.5340-5343.2003.View ArticlePubMedPubMed CentralGoogle Scholar
- Slavikova E, Vadkertiova R, Vranova D: Yeasts colonizing the leaf surfaces. J Basic Microbiol. 2007, 47 (4): 344-350. 10.1002/jobm.200710310.View ArticlePubMedGoogle Scholar
- Nova MX, Schuler AR, Brasileiro BT, Morais MA: Yeast species involved in artisanal cachaca fermentation in three stills with different technological levels in Pernambuco, Brazil. Food Microbiol. 2009, 26 (5): 460-466. 10.1016/j.fm.2009.02.005.View ArticlePubMedGoogle Scholar
- Li SS, Cheng C, Li Z, Chen JY, Yan B, Han BZ, Reeves M: Yeast species associated with wine grapes in China. Int J Food Microbiol. 2010, 138 (1–2): 85-90.View ArticlePubMedGoogle Scholar
- Comitini F, Gobbi M, Domizio P, Romani C, Lencioni L, Mannazzu I, Ciani M: Selected non-Saccharomyceswine yeasts in controlled multistarter fermentations with Saccharomyces cerevisiae. Food Microbiol. 2011, 28 (5): 873-882. 10.1016/j.fm.2010.12.001.View ArticlePubMedGoogle Scholar
- Maciel NO, Pilo FB, Freitas LF, Gomes FC, Johann S, Nardi RM, Lachance MA, Rosa CA: The diversity and antifungal susceptibility of the yeasts isolated from coconut water and reconstituted fruit juices in Brazil. Int J Food Microbiol. 2013, 160 (3): 201-205. 10.1016/j.ijfoodmicro.2012.10.012.View ArticlePubMedGoogle Scholar
- Kurtzman CP: Phylogenetic circumscription of Saccharomyces, Kluyveromycesand other members of the Saccharomycetaceae, and the proposal of the new genera Lachancea, Nakaseomyces, Naumovia, Vanderwaltozymaand Zygotorulaspora. FEMS Yeast Res. 2003, 4 (3): 233-245. 10.1016/S1567-1356(03)00175-2.View ArticlePubMedGoogle Scholar
- Naumova ES, Serpova EV, Naumov GI: Molecular systematics of Lachanceayeasts.Biochemistry (Mosc). 2007, 72 (12): 1356-1362. 10.1134/S0006297907120097.View ArticleGoogle Scholar
- Vuille C, Nidorf M, Weyman AE, Picard MH: Natural history of vegetations during successful medical treatment of endocarditis. Am Heart J. 1994, 128 (6 Pt 1): 1200-1209.View ArticlePubMedGoogle Scholar
- Queipo-Ortuno MI, Boto-Ordonez M, Murri M, Gomez-Zumaquero JM, Clemente-Postigo M, Estruch R, Cardona Diaz F, Andres-Lacueva C, Tinahones FJ: Influence of red wine polyphenols and ethanol on the gut microbiota ecology and biochemical biomarkers. Am J Clin Nutr. 2012, 95 (6): 1323-1334. 10.3945/ajcn.111.027847.View ArticlePubMedGoogle Scholar
- Choi G, Meijer SL, Hazenberg MD: Disseminated bread yeast fungaemia in a baker’s wife with acute myeloid leukaemia. Br J Haematol. 2012, 158 (3): 298-10.1111/j.1365-2141.2012.09212.x.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/250/prepub
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