Pulmonary immune responses to 2009 pandemic influenza A (H1N1) virus in mice
- Jin Lv†1, 2,
- Dan Wang†1,
- Yan-Hong Hua3,
- Shi-Jia Pei4,
- Jin Wang1,
- Wen-Wei Hu1,
- Xi-Liang Wang2Email author,
- Na Jia2Email author and
- Qi-Sheng Jiang1Email author
© Lv et al.; licensee BioMed Central Ltd. 2014
Received: 12 December 2013
Accepted: 7 April 2014
Published: 12 April 2014
Well-characterized mice models will afford a cheaper, easy-handling opportunity for a more comprehensive understanding of 2009 influenza A (H1N1) virus’s pathogenesis potential. We aimed to provide a robust description of pulmonary immune responses in the mice infected by the virus.
BALB/c mice were inoculated intranasally with A/Beijing/501/2009(H1N1) (BJ501) and A/PR/8/34(H1N1) (PR8) viruses and compared for survival rate, viral replication, and kinetics of pulmonary immune responses.
BJ501 virus replicated less efficiently in the lungs than PR8, and both caused lethal illness in the mice. The transient increases of pulmonary TNF-α 2 days post infection for BJ501 and of INF-γ and IL-10 at 6 days post infection for PR8 were observed. IL-2+ and IL-4+ secreting cells showed significant increase 12 days post infection, while IFN-γ+, IgG+ and IgA+ secreting cells increased 6 days post infection. The different patterns of pulmonary immunological parameters between two viruses were at most seen in IL-6, IL-17 secretion and IgG1/IgG2a ratio.
The BALB/c mouse is evaluated as a good pathogenic model for studying BJ501 2009 H1N1 virus. The work provided some basic and detailed data, which might be referred when further evaluating innate and adapted pulmonary immune responses and local viral load in mice.
KeywordsPandemic influenza Immunology BALB/c mice
During March and early April 2009, a sharp increase in patients with influenza-like illness and associated hospitalizations and deaths in several areas of Mexico was notified, which was later proved to be caused by a novel swine origin 2009 influenza A (H1N1) virus (2009 H1N1) . The virus was first identified in April from two unrelated pediatric febrile patients in southern California . Genetic analysis demonstrated the 2009 H1N1 virus contained a unique combination of gene segments from both North American and Eurasian swine lineages that has not been identified previously in either swine or human populations . By August 1, 2010, almost every country had reported laboratory confirmed cases, with over 18449 total death .
The main concerns about a new influenza pandemic are the disease burden and associated mortality it may cause. These are largely due to pneumonia resulting from viral infection. Many 2009 H1N1 infected patients have required hospitalization because of severe pneumonia and respiratory failure, with a fatal outcome . Previous study indicated that 2009 H1N1 virus replicated efficiently in the mice lungs and resulted lesions in the lung histologically [6–8]. These suggest the mice model might be proper for 2009 H1N1 virus infection without mouse adaptation.
2009 H1N1 virus has many different properties on its replication and induced pulmonary immune responses in the lungs. 2009 H1N1 virus caused more severe pulmonary lesion than seasonal influenza viruses in animals [8, 9]. In addition, the lower glycan binding properties also seemed to correlate with less-efficient transmission of 2009 H1N1 viruses in ferrets compared to seasonal H1N1 viruses . In addition, the pathogenesis of lung lesions caused by 2009 H1N1 viruses might be different from 1918 pandemic virus and highly pathogenic avian influenza H5N1 virus, as no major “cytokine storm” was observed with 2009 H1N1 infection of the human cell lines [10, 11] and the mice lungs . This lower cytokine production of 2009 H1N1 virus infection compared to 1918 virus and H5N1 virus might be associated with the findings that the virus did not spread to extrapulmonary organs and caused no viremia in ferrets or mice [8, 9]. Also, 2009 H1N1 virus differs in modest but subtle ways from seasonal influenza virus for cytokine dysregulation in vitro and in ex vivo cultures of human cell lines [10–12].
Thus, we want to study 2009 H1N1 virus pulmonary infection in the mice to provide a raw data on the Th1/Th2 cellular immune responses and IgG, IgA, IgM, and IgG subtype antibodies humoral immune responses at different days post infection.
A virulent strain of influenza A virus, A/PR/8/34(H1N1) (PR8), was kindly provided by Dr. Yuelong-Shu (Chinese Center for Disease Control and Prevention). Influenza H1N1 A/Beijing/501/2009(BJ501) virus was isolated and obtained from our institution. Virus stocks were propagated consistent with the original isolate passage in the allantoic cavity of 10-day-old embryonated hens’ eggs. Each egg was injected with 0.1 ml normal saline containing 1 haemagglutinin unit (HAU) of virus, incubated for 48 h at 34°C, and then held at 4°C overnight. The allantoic fluids were harvested, pooled, centrifuged to remove cellular debris and stored at −70°C until use. Virus stocks were low-passage-number viruses (E1-E2). For some assays, virus stock was further purified by centrifugation through a discontinuous sucrose gradient at 30,000 × g for 3 h at 4°C. The milky virus band located in the upper half of the gradient was harvested, washed in phosphate-buffered saline (PBS), and centrifuged at 24,000 × g for 4 h at 4°C. The virus pellet was gradually re-suspended in PBS, and aliquots were stored at −70°C. Virus stocks were quantified by a haemagglutination assay to obtain HAU. TCID50 was determined by titration in Madin-Darby canine kidney (MDCK) cells, with daily observation of cytopathic effect, and confirmed by haemagglutination assays. Viral titers were titrated by standard plaque assays in MDCK cells as previous described for determination of PFU titers . MDCK cells routinely maintained in Dulbecco’s Modified Eagle’s medium plus 10% fetal bovine serum (FBS). The 50% egg infectious dose (EID50) was determined by serial titration of virus stock in eggs, and EID50/ml values were calculated according to the method of Reed and Muench . All animal experiments with 2009 H1N1 viruses were conducted under biosafety level 3 enhanced (BSL3+) containment in accordance with guidelines of the World Health Organization .
Infection of mice
Female BALB/c mice (Institute of Jingfeng Medical Laboratory, Beijing, China), 6 to 8 weeks of age, were anesthetized with diethyl ether and inoculated intranasally (i.n.) with 25 μl of infectious virus diluted in sterile phosphate-buffered saline (PBS). Fifty-percent lethal dose (LD50) titers were determined by the inoculating groups of 6 mice i.n. with serial 10-fold dilutions of virus. LD50 titers were calculated by the method of Reed and Muench . For determination of lung virus titers, morbidity (measured by weight loss), and mortality at multiple time points during the two weeks of infection, 30 additional mice were infected i.n. with inoculating dose (5 × 105, 5 × 104 and 5 × 103 p.f.u.) of virus. Individual body weights from six mice were recorded for each group on days 0, 2, 4, 6, 8, 10, 12 and 14 post infections (p.i.) and monitored daily for disease signs and death for 14 days p.i. On days 2, 4, 6, 8, 10, 12 and 14 p.i., fifteen mice with inoculating dose (5 × 105 p.f.u.) of virus at each time point were euthanatized, lungs were collected separately and homogenized in 2 mL sterile PBS. After this process, homogenates were frozen separately in sterile tubes at −80°C for later titration of virus loads, antibody detection and cytokine determination. For the detection of lung immune cells and ELISPOT assay, eight to ten mice at each time point post infection were sacrificed by cervical dislocation. Single-cell suspensions of the lungs were prepared as follows. Freshly resected lungs were placed into cold DMEM, grinded gently and the suspensions were pelleted, resuspended and passed through nylon mesh filters. Red blood cells were removed by lysis buffer treatment (BD biosciences). Cells were counted and resuspended at appropriate concentrations for each particular experiment. The animal experiments were approved by the Animal Subjects Research Review Board of the Beijing Institute of Microbiology and Epidemiology and were conducted according to the institution's guidelines for animal husbandry.
Real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) detection of the viral load
The viral load in the lungs was determined by real time RT-PCR. RNA was extracted from the lungs by homogenizing with Trizol (Invitrogen, USA) reagent. Then, reverse transcription was performed by targeting a conserved region of the influenza matrix (M) gene for PR8 as previously described . For the detection of BJ501, the primers and probe were designed according to the CDC protocol for swine influenza A virus .
The lungs were harvested at various time points post infection. Single cell suspensions were stained with fluorochrome-labeled anti-CD3, anti-CD4, anti-CD8, anti-CD11b, and anti-CD11 cantibodies (BD Biosciences). Cells were labeled for 45 min at 4°C in staining buffer (PBS with 1% FBS, 0.02% NaN3) and then washed twice with PBS, and fixed overnight at 4°C with 2% paraformaldehyde. Flow cytometry was performed on a FACS Aria flow cytometer (BD Biosciences). The frequency of neutrophils (CD11b+/CD11c-/Ly6G/c+) was determined by appropriate gating within the total lung leukocytes. For determination of frequency of CD4+T cells and CD8+T cells, the CD3+T cells were first determined by appropriated gating within the pulmonary lymphocytes, and then CD4+/CD8−T cells and CD4−/CD8+T cells were detected by gating within the CD3+T cells.
ELISPOT (enzyme-linked immunospot) assay for Cytokine Secreting Cells (CSCs)
An ELISPOT assay was conducted as previously described . Briefly, ELISPOT 96-well plates (Millipore) were coated with anti-mouse IFN-γ, IL-2, and IL-4 capture antibody and incubated for 24 h at 4°C (BD Biosciences). The following day, the plates were washed and blocked with 200 μL of 1640 medium containing 10% fetal calf serum for 2 hours at room temperature to decrease the number of remaining protein-binding sites. Two hundred thousand cells of lung cell suspensions were added to each well and stimulated overnight at 37°C in 5% CO2 in the presence of RPMI 1640 (negative control), Con A (positive control), purified PR8 or BJ501 antigens (2.5 μg/ml). After 24 h of stimulation, the cells were washed and incubated for 24 h at 4°C with biotinylated anti-mouse IFN-γ, IL-2 and IL-4 antibodies (BD Biosciences). The plates were washed, and streptavidin-alkaline phosphatase (BD Biosciences) was added to each well and incubated for 2 h at room temperature. The plate was washed, 100 μl of AEC solution (BD Biosciences) were added to each well. The plate was then rinsed with distilled water and dried at room temperature. Spots were counted by an automated ELISPOT reader (CTL Limited).
ELISPOT assay for Antibody Secreting Cells (ASCs)
The numbers of influenza-virus-specific Antibody-Secreting Cells were determined using the ELISPOT assay, as described previously . Briefly, a 96-well plate with a nitrocellulose base (Millititer HA; Millipore) was coated with 2.5 μg/ml purified PR8 or BJ501 antigen, as an irrelevant-antigen control, wells were coated with phosphate-buffered saline containing 7.5 μg/mL BSA, then blocked with 200 μL of 1640 medium containing 10% fetal calf serum. A total of 5 × 106/ml cell suspensions from the lungs were added to each well and the mixture incubated for 9 h. The plate was washed with PBS–Tween and incubated with goat anti-mouse IgA (eBionsciences) or goat anti-mouse IgG (BD Biosciences) conjugated with biotin. Then, streptavidine conjugated with alkaline phosphatase (BD Biosciences) was added to each well and the plates were incubated for 1 hour at room temperature. The plates were washed as before with PBS-T and PBS and 100 μl/well of AEC solution were added. The reaction was stopped after 10–30 minutes at room temperature in dark by thorough rinsing with a tap water. Spots were counted by an automated ELISPOT reader (CTL Limited).
Influenza-specific pulmonary IgA, IgM and IgG isotypes antibodies were measured by enzyme-linked immunosorbent assay (ELISA), using plates coated with 1 μg/ml per well of purified PR8 or BJ501 influenza virus. The reactions were detected by goat anti-mouse IgA, IgM (Invitrogen), goat anti-mouse IgG1, IgG2b, IgG2a, IgG2c, and IgG3 (Bethyl laboratories, Inc.), goat anti-mouse IgG (Jackson ImmunoResearch Laboratories) antibodies conjugated to horseradish peroxidase. The plates were developed with TMB (Sigma, USA). Absorbance was read at 450 nm on a Bio-RAD model 550 Microplate Reader. The virus-specific Ab titer was defined as the reciprocal of the highest sample dilution giving an absorbance value greater than twice that of the samples from the negative controls. The titers were gained in duplicate.
The lungs were homogenized on ice individually in 2 ml PBS. Cytokine protein levels were measured by a specific ELISA assay following the kit description (R&D Systems, Minneapolis, MN, USA). The detection limits for the ELISA development kits are as follows: IFN-γ, 15-10000 pg/mL; TNF-α, 15-10000 pg/mL; IL-6: 15-10000 pg/mL; IL-17: 15-10000 pg/ml; IL-10, 15-2000 pg/mL.
Statistical differences at each time point within one group were determined by one-way ANOVA test, while statistical differences of the two viruses at the same time-point were determined by independent-sample T Test with SPSS19.0. Values of p<0.05 were considered significant.
Comparisons of survival rate, weight loss and viral load in the mice
The kinetics of pulmonary immune cell responses and cytokine productions in the mice
Antibody responses in the lung of mice
Influenza virus-specific CSCs and ASCs responses in the lungs of mice
To evaluate effective therapies and elucidate influenza pathogenesis, it is essential to have laboratory animal models that replicated the major features of illness in human. The animal models currently in use for influenza include mice, ferrets, rats , pigs , cats , dogs , and nonhuman primates . Mice and ferrets are currently the most intensively employed models, and mice remain the primary model due to their cost, inbreeding, and ability to utilize genetically modified animals. Influenza-virus-infected ferrets develop many of the typical signs of infection in humans . The limitations of the ferret model are the lack of specific reagents in studying immune system and the availability of animals without prior exposure to influenza . Our study demonstrated that 2009 H1N1 virus replicated efficiently in the lung of mice, and for severe infections and for lethal mice experiment as in this study, a high viral load might be present, and 2009 H1N1 virus seemed to induce a distinct pattern of cytokine markers associated with the pathogenesis.
The most outstanding cytokine is IL-6. Its correlation with severe infections of 2009 H1N1 virus was demonstrated in both human cases [27, 28] and in high dose inoculated mice model . In our mice experiment, we found IL-6 significantly increased not only in the early but also in the late stage of infection. IL-6, a multifunctional cytokine expressed by both lymphoid and non-lymphoid cells , played a central role in resolving the innate immune response and directing the transition from innate to adaptive immunity , and IL-6 concentrations were significantly correlated with the symptoms and signs of influenza A infection in human [31, 32], whereas depletion of IL-6 did not directly protect against death from lethal H5N1 influenza virus infection . Recently, IL-6 is found to be in the regulation of Th17 differentiation . Th17 cell also secrets cytokine of IL-17, which is critical in the immunopathology of acute lung injury due to acute influenza infection .
However, the in vivo role of IL-17 in the clearance of influenza virus and its immune pathology has been controversial. Several studies have demonstrated the important protective role of IL-17 against lethal influenza infection. In contrast, other groups have showed that IL-17 signaling was not required for viral clearance, but rather played a pathogenic role by recruiting neutrophils to the site of inflammation [27, 36–38]. Furthermore, IL-17 deficiency or treatment with monoclonal antibodies against IL-17-ameliorated acute lung injury induced by the 2009 H1N1 virus in the mice . In our mice experiment, we found significant decreased pulmonary IL-17 both in BI501 and PR8 infection, whether this correlated with the virus replication in local deserved further study.
TNF-α is an important proinflammatory cytokine located at the downstream of IL-17. Previous study found it also had a positive feedback effect on Th17 in vitro. The transient increase of TNF-α at days 2 might contribute in the enhancement of chemokine gene expression, macrophages and dendritic cells maturation, and further activation of antigen presentation . TNF-α level has been found to correlate directly with the severity of gross and histologic lung lesions in the influenza infected mice . Daniela Damjanovic and his colleagues suggested that TNF-α was dispensable for influenza clearance. Their study showed that TNF-α was up-regulated in the lung after influenza infection and TNF-α deficiency led not only to a greater extent of illness but also to heightened lung immunopathology and tissue remodeling . Taken together, proinflammatory cytokine (TNF-α) and mediators involved in the development of Th17 (IL-17, IL-6) responses were associated with the critical illness of 2009 pandemic influenza infection either in the detrimental or beneficial role. The IL-6 pathway had been a promising new therapeutic target in treatment of autoimmune illness [44, 45]. The use of anti-inflammatory and immunomodulatory agents for pandemic influenza control should be largely evaluated in case of antiviral resistance .
Several studies had indicated that the increased pathogenicity of influenza A virus may be associated with extremely rapid recruitment of macrophages and neutrophils into the lungs in fatal infection [47, 48]. In addition, in the late recovery phase, macrophage and neutrophils may be in primary cooperation with the antibody working at the late-phase clearance of influenza virus through phagocytic elimination of infected cells [49, 50]. Contrast to IFN-γ+ CSCs increasing from the early infection, IL-2+ and IL-4+ CSCs only increased 12 days post infection, which implied the strongly stimulated adapted immune response. In addition, we found that BJ501 virus also induced relatively stronger Th2 mediated responses (pulmonary IgA+ and IgG+ secreting cells, IgG2/IgG2a ratio) in the lung than PR8 virus. These stronger Th2 immune responses might be correlated with the relatively less efficient virus replication in the lung.
Amino acid differences among BJ 501, Cal 04 and PR 8 influenza viruses
−627 K associated with increased transmission, advantageous replication at 33°C and confers air transmission of avian virus.
-Important determination of host range. Avian strains have 627E, human strains 627 K.
-627 K is linked to increased virulence in mice.
−42S is critical for virulence of H5N1 strains and to antagonize host cell interferon induction
-Oseltamivir resistance is associated to H274Y
-Confers cross-resistance to adamantane drugs
Although the global wave of 2009 H1N1 virus has went away, many unsolved questions are still important to be further clarified for prevention and control of potential pandemic threat. This study provided some basic data on the optimal time to observe different immune parameters, for example, IL-2+, IL-4+ CSCs stimulated about 12 days post infection, TNF-α, IFN-γ, IL-10 cytokines only transiently increased in the early infection. It is hoped this paper will contribute for immunological researchers to the better use of mice experiment to study the correlation between the viral load and critical immune response in the lung.
This study was supported by the grants of National Natural Science Foundation of China (81172130).
- Chowell G, Bertozzi SM, Colchero MA, Lopez-Gatell H, Alpuche-Aranda C, Hernandez M, Miller MA: Severe respiratory disease concurrent with the circulation of H1N1 influenza. N Engl J Med. 2009, 361 (7): 674-679. 10.1056/NEJMoa0904023.View ArticlePubMedGoogle Scholar
- Peiris JS, Poon LL, Guan Y: Emergence of a novel swine-origin influenza A virus (S-OIV) H1N1 virus in humans. J Clin Virol. 2009, 45 (3): 169-173. 10.1016/j.jcv.2009.06.006.View ArticlePubMedPubMed CentralGoogle Scholar
- Garten RJ, Davis CT, Russell CA, Shu B, Lindstrom S, Balish A, Sessions WM, Xu X, Skepner E, Deyde V, Okomo-Adhiambo M, Gubareva L, Barnes J, Smith CB, Emery SL, Hillman MJ, Rivailler P, Smagala J, de Graaf M, Burke DF, Fouchier RA, Pappas C, Alpuche-Aranda CM, Lopez-Gatell H, Olivera H, Lopez I, Myers CA, Faix D, Blair PJ, Yu C: Antigenic and genetic characteristics of swine-origin 2009 A(H1N1) influenza viruses circulating in humans. Science. 2009, 325 (5937): 197-201. 10.1126/science.1176225.View ArticlePubMedPubMed CentralGoogle Scholar
- 2010, 08 (06): 06-http://www.who.int/csr/don/2010_08_06/en/index.html,
- Davies A, Jones D, Bailey M, Beca J, Bellomo R, Blackwell N, Forrest P, Gattas D, Granger E, Herkes R, Jackson A, McGuinness S, Nair P, Pellegrino V, Pettila V, Plunkett B, Pye R, Torzillo P, Webb S, Wilson M, Ziegenfuss M: Extracorporeal Membrane Oxygenation for 2009 Influenza A(H1N1) Acute Respiratory Distress Syndrome. Jama. 2009, 302 (17): 1888-1895.View ArticlePubMedGoogle Scholar
- Belser JA, Wadford DA, Pappas C, Gustin KM, Maines TR, Pearce MB, Zeng H, Swayne DE, Pantin-Jackwood M, Katz JM, Tumpey TM: Pathogenesis of pandemic influenza A (H1N1) and triple-reassortant swine influenza A (H1) viruses in mice. J Virol. 2010, 84 (9): 4194-4203. 10.1128/JVI.02742-09.View ArticlePubMedPubMed CentralGoogle Scholar
- Itoh Y, Shinya K, Kiso M, Watanabe T, Sakoda Y, Hatta M, Muramoto Y, Tamura D, Sakai-Tagawa Y, Noda T, Sakabe S, Imai M, Hatta Y, Watanabe S, Li C, Yamada S, Fujii K, Murakami S, Imai H, Kakugawa S, Ito M, Takano R, Iwatsuki-Horimoto K, Shimojima M, Horimoto T, Goto H, Takahashi K, Makino A, Ishigaki H, Nakayama M: In vitro and in vivo characterization of new swine-origin H1N1 influenza viruses. Nature. 2009, 460 (7258): 1021-1025.PubMedPubMed CentralGoogle Scholar
- Maines TR, Jayaraman A, Belser JA, Wadford DA, Pappas C, Zeng H, Gustin KM, Pearce MB, Viswanathan K, Shriver ZH, Raman R, Cox NJ, Sasisekharan R, Katz JM, Tumpey TM: Transmission and pathogenesis of swine-origin 2009 A(H1N1) influenza viruses in ferrets and mice. Science. 2009, 325 (5939): 484-487.PubMedPubMed CentralGoogle Scholar
- Munster VJ, De Wit E, van den Brand JM, Herfst S, Schrauwen EJ, Bestebroer TM, van de Vijver D, Boucher CA, Koopmans M, Rimmelzwaan GF, Kuiken T, Osterhaus AD, Fouchier RA: Pathogenesis and transmission of swine-origin 2009 A(H1N1) influenza virus in ferrets. Science. 2009, 325 (5939): 481-483.PubMedPubMed CentralGoogle Scholar
- Osterlund P, Pirhonen J, Ikonen N, Ronkko E, Strengell M, Makela SM, Broman M, Hamming OJ, Hartmann R, Ziegler T, Julkunen I: Pandemic H1N1 2009 influenza A virus induces weak cytokine responses in human macrophages and dendritic cells and is highly sensitive to the antiviral actions of interferons. J Virol. 2010, 84 (3): 1414-1422. 10.1128/JVI.01619-09.View ArticlePubMedGoogle Scholar
- Woo PC, Tung ET, Chan KH, Lau CC, Lau SK, Yuen KY: Cytokine profiles induced by the novel swine-origin influenza A/H1N1 virus: implications for treatment strategies. J Infect Dis. 2010, 201 (3): 346-353. 10.1086/649785.View ArticlePubMedGoogle Scholar
- Chan MC, Chan RW, Yu WC, Ho CC, Yuen KM, Fong JH, Tang LL, Lai WW, Lo AC, Chui WH, Sihoe AD, Kwong DL, Wong DS, Tsao GS, Poon LL, Guan Y, Nicholls JM, Peiris JS: Tropism and innate host responses of the 2009 pandemic H1N1 influenza virus in ex vivo and in vitro cultures of human conjunctiva and respiratory tract. Am J Pathol. 2010, 176 (4): 1828-1840. 10.2353/ajpath.2010.091087.View ArticlePubMedPubMed CentralGoogle Scholar
- Zeng H, Goldsmith C, Thawatsupha P, Chittaganpitch M, Waicharoen S, Zaki S, Tumpey TM, Katz JM: Highly pathogenic avian influenza H5N1 viruses elicit an attenuated type i interferon response in polarized human bronchial epithelial cells. J Virol. 2007, 81 (22): 12439-12449. 10.1128/JVI.01134-07.View ArticlePubMedPubMed CentralGoogle Scholar
- Thakur AK, Fezio WL: A computer program for estimating LD50 and its confidence limits using modified Behrens-Reed-Muench cumulant method. Drug Chem Toxicol. 1981, 4 (3): 297-305. 10.3109/01480548109018136.View ArticlePubMedGoogle Scholar
- WHO: http://www.who.int/csr/resources/publications/biosafety/WHO_CDS_EPR_2006_6.pdf?ua=1,
- Jelley-Gibbs DM, Dibble JP, Brown DM, Strutt TM, McKinstry KK, Swain SL: Persistent depots of influenza antigen fail to induce a cytotoxic CD8 T cell response. J Immunol. 2007, 178 (12): 7563-7570. 10.4049/jimmunol.178.12.7563.View ArticlePubMedGoogle Scholar
- Bolotin S, Robertson AV, Eshaghi A, De Lima C, Lombos E, Chong-King E, Burton L, Mazzulli T, Drews SJ: Development of a novel real-time reverse-transcriptase PCR method for the detection of H275Y positive influenza A H1N1 isolates. J Virol Methods. 2009, 158 (1–2): 190-194.View ArticlePubMedGoogle Scholar
- Kutzler MA, Robinson TM, Chattergoon MA, Choo DK, Choo AY, Choe PY, Ramanathan MP, Parkinson R, Kudchodkar S, Tamura Y, Sidhu M, Roopchand V, Kim JJ, Pavlakis GN, Felber BK, Waldmann TA, Boyer JD, Weiner DB: Coimmunization with an optimized IL-15 plasmid results in enhanced function and longevity of CD8 T cells that are partially independent of CD4 T cell help. J Immunol. 2005, 175 (1): 112-123. 10.4049/jimmunol.175.1.112.View ArticlePubMedGoogle Scholar
- Manz RA, Arce S, Cassese G, Hauser AE, Hiepe F, Radbruch A: Humoral immunity and long-lived plasma cells. Curr Opin Immunol. 2002, 14 (4): 517-521. 10.1016/S0952-7915(02)00356-4.View ArticlePubMedGoogle Scholar
- Daniels MJ, Selgrade MK, Doerfler D, Gilmour MI: Kinetic profile of influenza virus infection in three rat strains. Comp Med. 2003, 53 (3): 293-298.PubMedGoogle Scholar
- van der Laan JW, Herberts C, Lambkin-Williams R, Boyers A, Mann AJ, Oxford J: Animal models in influenza vaccine testing. Expert Rev Vaccines. 2008, 7 (6): 783-793. 10.1586/147605184.108.40.2063.View ArticlePubMedGoogle Scholar
- Vahlenkamp TW, Harder TC, Giese M, Lin F, Teifke JP, Klopfleisch R, Hoffmann R, Tarpey I, Beer M, Mettenleiter TC: Protection of cats against lethal influenza H5N1 challenge infection. J Gen Virol. 2008, 89 (Pt 4): 968-974.View ArticlePubMedGoogle Scholar
- Giese M, Harder TC, Teifke JP, Klopfleisch R, Breithaupt A, Mettenleiter TC, Vahlenkamp TW: Experimental infection and natural contact exposure of dogs with avian influenza virus (H5N1). Emerg Infect Dis. 2008, 14 (2): 308-310. 10.3201/eid1402.070864.View ArticlePubMedPubMed CentralGoogle Scholar
- Baskin CR, Garcia-Sastre A, Tumpey TM, Bielefeldt-Ohmann H, Carter VS, Nistal-Villan E, Katze MG: Integration of clinical data, pathology, and cDNA microarrays in influenza virus-infected pigtailed macaques (Macaca nemestrina). J Virol. 2004, 78 (19): 10420-10432. 10.1128/JVI.78.19.10420-10432.2004.View ArticlePubMedPubMed CentralGoogle Scholar
- Svitek N, Rudd PA, Obojes K, Pillet S, Von Messling V: Severe seasonal influenza in ferrets correlates with reduced interferon and increased IL-6 induction. Virology. 2008, 376 (1): 53-59. 10.1016/j.virol.2008.02.035.View ArticlePubMedGoogle Scholar
- Barnard DL: Animal models for the study of influenza pathogenesis and therapy. Antiviral Res. 2009, 82 (2): A110-A122. 10.1016/j.antiviral.2008.12.014.View ArticlePubMedPubMed CentralGoogle Scholar
- Bermejo-Martin JF, Ortiz De Lejarazu R, Pumarola T, Rello J, Almansa R, Ramirez P, Martin-Loeches I, Varillas D, Gallegos MC, Seron C, Micheloud D, Gomez JM, Tenorio-Abreu A, Ramos MJ, Molina ML, Huidobro S, Sanchez E, Gordon M, Fernandez V, Del Castillo A, Marcos MA, Villanueva B, Lopez CJ, Rodriguez-Dominguez M, Galan JC, Canton R, Lietor A, Rojo S, Eiros JM, Hinojosa C: Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza. Crit Care. 2009, 13 (6): R201-10.1186/cc8208.View ArticlePubMedPubMed CentralGoogle Scholar
- Giamarellos-Bourboulis EJ, Raftogiannis M, Antonopoulou A, Baziaka F, Koutoukas P, Savva A, Kanni T, Georgitsi M, Pistiki A, Tsaganos T, Pelekanos N, Athanassia S, Galani L, Giannitsioti E, Kavatha D, Kontopidou F, Mouktaroudi M, Poulakou G, Sakka V, Panagopoulos P, Papadopoulos A, Kanellakopoulou K, Giamarellou H: Effect of the novel influenza A (H1N1) virus in the human immune system. PLoS One. 2009, 4 (12): e8393-10.1371/journal.pone.0008393.View ArticlePubMedPubMed CentralGoogle Scholar
- Kishimoto T: Interleukin-6: from basic science to medicine–40 years in immunology. Annu Rev Immunol. 2005, 23: 1-21. 10.1146/annurev.immunol.23.021704.115806.View ArticlePubMedGoogle Scholar
- Jones SA: Directing transition from innate to acquired immunity: defining a role for IL-6. J Immunol. 2005, 175 (6): 3463-3468. 10.4049/jimmunol.175.6.3463.View ArticlePubMedGoogle Scholar
- Hayden FG, Fritz R, Lobo MC, Alvord W, Strober W, Straus SE: Local and systemic cytokine responses during experimental human influenza A virus infection. Relation to symptom formation and host defense. J Clin Invest. 1998, 101 (3): 643-649. 10.1172/JCI1355.View ArticlePubMedPubMed CentralGoogle Scholar
- Skoner DP, Gentile DA, Patel A, Doyle WJ: Evidence for cytokine mediation of disease expression in adults experimentally infected with influenza A virus. J Infect Dis. 1999, 180 (1): 10-14. 10.1086/314823.View ArticlePubMedGoogle Scholar
- Salomon R, Hoffmann E, Webster RG: Inhibition of the cytokine response does not protect against lethal H5N1 influenza infection. Proc Natl Acad Sci U S A. 2007, 104 (30): 12479-12481. 10.1073/pnas.0705289104.View ArticlePubMedPubMed CentralGoogle Scholar
- Chen Z, Laurence A, O'Shea JJ: Signal transduction pathways and transcriptional regulation in the control of Th17 differentiation. Semin Immunol. 2007, 19 (6): 400-408. 10.1016/j.smim.2007.10.015.View ArticlePubMedPubMed CentralGoogle Scholar
- Crowe CR, Chen K, Pociask DA, Alcorn JF, Krivich C, Enelow RI, Ross TM, Witztum JL, Kolls JK: Critical role of IL-17RA in immunopathology of influenza infection. J Immunol. 2009, 183 (8): 5301-5310. 10.4049/jimmunol.0900995.View ArticlePubMedPubMed CentralGoogle Scholar
- Norton EB, Clements JD, Voss TG, Cardenas-Freytag L: Prophylactic administration of bacterially derived immunomodulators improves the outcome of influenza virus infection in a murine model. J Virol. 2010, 84 (6): 2983-2995. 10.1128/JVI.01805-09.View ArticlePubMedPubMed CentralGoogle Scholar
- Pelletier M, Maggi L, Micheletti A, Lazzeri E, Tamassia N, Costantini C, Cosmi L, Lunardi C, Annunziato F, Romagnani S, Cassatella MA: Evidence for a cross-talk between human neutrophils and Th17 cells. Blood. 2010, 115 (2): 335-343. 10.1182/blood-2009-04-216085.View ArticlePubMedGoogle Scholar
- Linden A, Laan M, Anderson GP: Neutrophils, interleukin-17A and lung disease. Eur Respir J. 2005, 25 (1): 159-172. 10.1183/09031936.04.00032904.View ArticlePubMedGoogle Scholar
- Li C, Yang P, Sun Y, Li T, Wang C, Wang Z, Zou Z, Yan Y, Wang W, Wang C, Chen Z, Xing L, Tang C, Ju X, Guo F, Deng J, Zhao Y, Yang P, Tang J, Wang H, Zhao Z, Yin Z, Cao B, Wang X, Jiang C: IL-17 response mediates acute lung injury induced by the 2009 pandemic influenza A (H1N1) virus. Cell Res. 2012, 22 (3): 528-538. 10.1038/cr.2011.165.View ArticlePubMedGoogle Scholar
- Sutton C, Brereton C, Keogh B, Mills KH, Lavelle EC: A crucial role for interleukin (IL)-1 in the induction of IL-17-producing T cells that mediate autoimmune encephalomyelitis. J Exp Med. 2006, 203 (7): 1685-1691. 10.1084/jem.20060285.View ArticlePubMedPubMed CentralGoogle Scholar
- Julkunen I, Sareneva T, Pirhonen J, Ronni T, Melen K, Matikainen S: Molecular pathogenesis of influenza A virus infection and virus-induced regulation of cytokine gene expression. Cytokine Growth Factor Rev. 2001, 12 (2–3): 171-180.View ArticlePubMedGoogle Scholar
- Peper RL, Van Campen H: Tumor necrosis factor as a mediator of inflammation in influenza A viral pneumonia. Microb Pathog. 1995, 19 (3): 175-183. 10.1006/mpat.1995.0056.View ArticlePubMedGoogle Scholar
- Damjanovic D, Divangahi M, Kugathasan K, Small CL, Zganiacz A, Brown EG, Hogaboam CM, Gauldie J, Xing Z: Negative regulation of lung inflammation and immunopathology by TNF-alpha during acute influenza infection. Am J Pathol. 2011, 179 (6): 2963-2976. 10.1016/j.ajpath.2011.09.003.View ArticlePubMedPubMed CentralGoogle Scholar
- Cash H, Relle M, Menke J, Brochhausen C, Jones SA, Topley N, Galle PR, Schwarting A: Interleukin 6 (IL-6) deficiency delays lupus nephritis in MRL-Faslpr mice: the IL-6 pathway as a new therapeutic target in treatment of autoimmune kidney disease in systemic lupus erythematosus. J Rheumatol. 2010, 37 (1): 60-70. 10.3899/jrheum.090194.View ArticlePubMedGoogle Scholar
- Yue C, You X, Zhao L, Wang H, Tang F, Zhang F, Zhang X, He W: The effects of adalimumab and methotrexate treatment on peripheral Th17 cells and IL-17/IL-6 secretion in rheumatoid arthritis patients. Rheumatol Int. 2010, 30 (12): 1553-1557. 10.1007/s00296-009-1179-x.View ArticlePubMedGoogle Scholar
- Fedson DS: Confronting an influenza pandemic with inexpensive generic agents: can it be done?. Lancet Infect Dis. 2008, 8 (9): 571-576. 10.1016/S1473-3099(08)70070-7.View ArticlePubMedGoogle Scholar
- Perrone LA, Plowden JK, Garcia-Sastre A, Katz JM, Tumpey TM: H5N1 and 1918 pandemic influenza virus infection results in early and excessive infiltration of macrophages and neutrophils in the lungs of mice. PLoS Pathog. 2008, 4 (8): e1000115-10.1371/journal.ppat.1000115.View ArticlePubMedPubMed CentralGoogle Scholar
- Suzuki K, Okada H, Itoh T, Tada T, Mase M, Nakamura K, Kubo M, Tsukamoto K: Association of increased pathogenicity of Asian H5N1 highly pathogenic avian influenza viruses in chickens with highly efficient viral replication accompanied by early destruction of innate immune responses. J Virol. 2009, 83 (15): 7475-7486. 10.1128/JVI.01434-08.View ArticlePubMedPubMed CentralGoogle Scholar
- Fujisawa H: Neutrophils play an essential role in cooperation with antibody in both protection against and recovery from pulmonary infection with influenza virus in mice. J Virol. 2008, 82 (6): 2772-2783. 10.1128/JVI.01210-07.View ArticlePubMedPubMed CentralGoogle Scholar
- Hashimoto Y, Moki T, Takizawa T, Shiratsuchi A, Nakanishi Y: Evidence for phagocytosis of influenza virus-infected, apoptotic cells by neutrophils and macrophages in mice. J Immunol. 2007, 178 (4): 2448-2457. 10.4049/jimmunol.178.4.2448.View ArticlePubMedGoogle Scholar
- Massin P, van der Werf S, Naffakh N: Residue 627 of PB2 is a determinant of cold sensitivity in RNA replication of avian influenza viruses. J Virol. 2001, 75 (11): 5398-5404. 10.1128/JVI.75.11.5398-5404.2001.View ArticlePubMedPubMed CentralGoogle Scholar
- Soundararajan V, Tharakaraman K, Raman R, Raguram S, Shriver Z, Sasisekharan V, Sasisekharan R: Extrapolating from sequence–the 2009 H1N1 'swine' influenza virus. Nat Biotechnol. 2009, 27 (6): 510-513. 10.1038/nbt0609-510.View ArticlePubMedGoogle Scholar
- Arias CF, Escalera-Zamudio M, Soto-Del Rio Mde L, Cobian-Guemes AG, Isa P, Lopez S: Molecular anatomy of 2009 influenza virus A (H1N1). Arch Med Res. 2009, 40 (8): 643-654. 10.1016/j.arcmed.2009.10.007.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/14/197/prepub
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