Volume 12 Supplement 1
Association of IFN-γ gene polymorphism and levels in HBV related disease chronicity in India
© Saxena et al; licensee BioMed Central Ltd. 2012
Published: 4 May 2012
Hepatitis B Virus (HBV) infection is a primary causative factor for hepatocellular carcinoma (HCC), the fifth most frequent cancer, worldwide. The present study evaluated the association of IFN-γ +874T>A polymorphism and its levels with HBV related HCC risk in Indian population.
Five groups of subjects were enrolled viz. control (n=146), HBV-carriers (n=68), chronic active HBV (n=64), HBV-cirrhotics (n=60) and HBV-related HCC (n=59). Allele-specific-PCR was performed to study various polymorphic forms of IFN-γ (+874) and blood levels were estimated by ELISA. Genotype distribution was compared using chi square analysis and the odds ratios (ORs) and 95% CI were calculated to express the relative risk.
In IFN-γ (+874), the (TA) heterozygous genotype was found to be a significant risk factor for chronic-active hepatitis (OR=2.94, p<0.01) and HCC (OR=2.7, p<0.01) development, among controls and carrier groups, respectively. Similarly, the variant (AA) genotype, was also found to be significantly in positive association with HCC risk (p<0.01), among controls. Further, the spontaneous median IFN-γ levels were 151.09pg/mL in control population. While, the levels were significantly elevated (p<0.01) in HCC group (252.34pg/mL) in comparison to controls as well as other categories studied. However, no significant association was found between the genotype and the cytokine levels.
IFN-γ polymorphic forms and its levels share a strong association with HBV-HCC risk in Indian population and thus, should be further evaluated as a candidate gene to determine individual susceptibility for the same.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.