Volume 12 Supplement 1
Detection of norovirus in stool samples by RT-PCR in 5 disease centers in Iran
© Adabian et al; licensee BioMed Central Ltd. 2012
Published: 4 May 2012
Noroviruses are a group of viruses that cause gastroenteritis (illness that usually includes diarrhea and/or vomiting) in people. Gastroenteritis means inflammation of the stomach and small and large intestines. Many different viruses can cause gastroenteritis, including rotaviruses; noroviruses; adenoviruses, types 40 and 41; sapoviruses; and astroviruses. Current techniques used for detection of noroviruses in stool samples include multi-step viral RNA extraction and purification followed by reverse transcriptase-polymerase chain reaction (RT-PCR). The aim of this study is the detection of norovirus in stool samples by RT-PCR in 5 disease centers in Iran.
In this descriptive study, 2170 stool samples of patients consulting for acute gastroenteritis at a pediatric hospital in 5 cities of Iran were enrolled. The mean age of the study population was 48 months with an age range of 30 days to 4 years. Fecal specimens were collected within 24hrs of admission. The specimens were frozen, sent to the laboratory, and then stored at -80°C until being tested for norovirus.
RT-PCR was evaluated with 2170 stool samples containing 90 (4.14%) norovirus-positive (0.97% Tehran, 0.64% Tabriz, 0.18% Mashhad, 1.57% Shiraz, 0.78% Bandar Abbas). The RT-PCR was validated with published primers for norovirus (JV12/JV13). In both retrospective and prospective settings, the RT-PCR was equally sensitive and specific in detecting norovirus.
Acute gastroenteritis can be caused by norovirus. It has to be attending to vaccination against norovirus after rotavirus.
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.