Bacterial infection profiles in lung cancer patients with febrile neutropenia
© Lanoix et al; licensee BioMed Central Ltd. 2011
Received: 7 January 2011
Accepted: 27 June 2011
Published: 27 June 2011
The chemotherapy used to treat lung cancer causes febrile neutropenia in 10 to 40% of patients. Although most episodes are of undetermined origin, an infectious etiology can be suspected in 30% of cases. In view of the scarcity of data on lung cancer patients with febrile neutropenia, we performed a retrospective study of the microbiological characteristics of cases recorded in three medical centers in the Picardy region of northern France.
We analyzed the medical records of lung cancer patients with neutropenia (neutrophil count < 500/mm3) and fever (temperature > 38.3°C).
The study included 87 lung cancer patients with febrile neutropenia (mean age: 64.2). Two thirds of the patients had metastases and half had poor performance status. Thirty-three of the 87 cases were microbiologically documented. Gram-negative bacteria (mainly enterobacteriaceae from the urinary and digestive tracts) were identified in 59% of these cases. Staphylococcus species (mainly S. aureus) accounted for a high proportion of the identified Gram-positive bacteria. Bacteremia accounted for 60% of the microbiologically documented cases of fever. 23% of the blood cultures were positive. 14% of the infections were probably hospital-acquired and 14% were caused by multidrug-resistant strains. The overall mortality rate at day 30 was 33% and the infection-related mortality rate was 16.1%. Treatment with antibiotics was successful in 82.8% of cases. In a multivariate analysis, predictive factors for treatment failure were age >60 and thrombocytopenia < 20000/mm3.
Gram-negative species were the most frequently identified bacteria in lung cancer patients with febrile neutropenia. Despite the success of antibiotic treatment and a low-risk neutropenic patient group, mortality is high in this particular population.
Lung cancer is the most frequent and severe cancer in France. In 2000, the incidence was 52.2 per 100,000 inhabitants per year . Although lung cancer mainly affects male, middle-aged smokers, the recent increase in the number of female smokers is changing these data . The choice of treatment depends on the lung cancer's histological characteristics. When chemotherapy is used, neutropenia (i.e. a neutrophil count < 500/mm3) of variable severity and duration occurs in 10 to 40% of patients (depending on the type of chemotherapy) [3, 4]. In solid tumors, neutropenia usually lasts for less than 7 days. However, the condition can be complicated by fever (a body temperature of over 38.3°C once an hour or over 38°C twice an hour) in 8 to 15% of cases and is thus referred to as febrile neutropenia [5, 6]. Microbiological evidence is found in only 30% of patients with neutropenic fever. Indeed, approximately 60% of patients with febrile neutropenia have neither clinical signs nor microbiological evidence of infection .
Prior to the 1980s, neutropenic fever was due to Gram-negative bacteria (e.g. Escherichia coli, Klebsiella sp, Pseudomonas aeruginosa, etc.) in 70% of cases [8, 9]. Since then, the proportion of Gram-positive bacteria has risen, with Staphylococcus epidermidis being found more frequently than Staphylococcus aureus .
Lung cancer patients are characterized by the frequent presence of Pseudomonas aeruginosa in chronic obstructive pulmonary disease, together with age-related comorbidities, aggressive tumors and rapid clinical degradation.
Markman and Abeloff (1983) studied the infectious complications of chemotherapy (with cyclophosphamide, doxorubicin and etoposide) in 71 patients with small-cell carcinoma . Although 90% of cases were complicated by febrile neutropenia, an etiology was determined in only 20% of the latter. The following bacteria were found: Escherichia coli (n = 3), Streptococcus viridans (n = 2), Pseudomonas aeruginosa (n = 2), Bacillus sp. (n = 2), Staphylococcus aureus (n = 1), Candida sp. (n = 1), Enterococcus sp. (n = 1) and one anaerobic Gram-negative bacillus. None of these cases of febrile neutropenia resulted in death.
Two other studies in lung cancer patients examined the outcomes of antibiotic therapy rather than the microbiological characteristics of the condition [12, 13]. No statistically significant differences were found.
Here, we performed a retrospective, descriptive, multicenter study of the microbiological characteristics of febrile neutropenia in lung cancer patients hospitalized in three medical centers in the Picardy region of northern France. The study's primary objective was to determine risk factors for antibiotic treatment failure.
We included adult lung cancer patients (i.e. aged 18 or over) having undergone chemotherapy (of whatever type) between January 2000 and July 2006 in three medical centers in the Picardy region. Patients had to have displayed concomitant neutropenia (i.e. a neutrophil count < 500/mm3) and fever (a body temperature > 38.3°C once an hour or over 38°C twice an hour). Access to the patients' medical records was provided by the medical centers' health informatics departments.
We excluded patients who had already experienced an episode of febrile neutropenia prior to study entry, patients with cancers other than lung cancer, cases of fever without neutropenia, cases of neutropenia without fever and cases of fever with a clearly non-infectious etiology.
Data was recorded in a pre-determined questionnaire. Febrile episodes were classified according to Hughes' criteria as being microbiologically documented, clinically documented or of undetermined origin. The success of antibiotic therapy was defined according to Kern's criteria: no fever for 3 successive days, the absence of clinical signs or the eradication of an identified pathogen. The death of a patient meeting these criteria was not considered to be a treatment failure . The last criterion for success was the lack of recurrence within 7 days of the end of antibiotic therapy. We calculated the "first-line antibiotic treatment failure rate" (i.e. failure of the first-line antibiotic prescribed) and the "overall antibiotic treatment success rate" (i.e. a successful outcome when one or more antibiotics had been prescribed).
We also recorded the presence of the following severity criteria on admission: systolic blood pressure < 90 mmHg, blood oxygen partial pressure < 60 mmHg, disseminated intravascular coagulation, confusion, cardiac failure, blood transfusion for hemorrhage, kidney failure and admission to the intensive care unit .
Data were gathered in compliance with the principles of the Declaration of Helsinki and then anonymized. No patient consent was needed because of the confidential nature of this study, which was approved by the independent ethics committee at Saint-Quentin Medical Center.
Statistical analysis was performed with Excel® 2000 (Microsoft EMEA, Courtaboeuf, France) and SPSS (SPSS Inc., Chicago, IL) software. Quantitative variables were expressed as the mean ± standard deviation and qualitative variables were expressed as the frequency and percentage. Univariate, comparative analyses were performed by using Fisher's exact test (for qualitative variables) and the Mann-Whitney test (for quantitative variables). The threshold for statistical significance was set to p < 0.05. Multivariate analyses were performed by using logistic regression.
Characteristics of patients
Number (%)or means ± SD
64.2 ± 9.6
Score WHO ≥ 2
- therapy by corticosteroids
- other cancer
- other comorbidity
No of comorbidities
1.3 ± 0.5
No. of comorbidities ≥ 2
No of packets × No of year of smoking
46.5 ± 19.5
History of pulmonary diseases
- restrictive syndrome
- pulmonary tuberculosis
- FEV1 > 80% of theory
- FEV1 between 50 and 80% theory
- FEV1 between 30 and 50% theory
- FEV1 < 30% of theory
Almost two thirds of the patients had metastatic cancer when they were diagnosed with febrile neutropenia (62% in all, including brain metastases in 14% and metastases at more than two sites in 20%). Thirty-one percent of the non-metastatic patients were non-operable and 28.7% (n = 25) of patients had also been treated with radiotherapy (data not shown). Eighty patients (92%) had an implantable venous access system and 15 patients (17%) had undergone two or more cycles of chemotherapy.
Thirteen percent of the patients had febrile neutropenia (87 out of 646). Sixteen events were excluded because they did not correspond to the patient's first ever occurrence of febrile neutropenia. The mean duration of neutropenia was 4.4 ± 2.4 days (range: 1-11) and 10% of the episodes lasted more than 8 days.
The fever was classified as being of undetermined origin in 46% of cases (n = 40), clinically documented in 45% of cases (n = 39) (including 14 (16%) with no microbiology documentation) and microbiologically documented in 38% of cases (n = 33). Three quarters of the microbiologically documented cases (n = 25 out of 33) were also clinically documented. The clinically documented infections were classified as follows: 21 lower respiratory tract infections (53%), 9 digestive infections (23%), 3 catheter infections (8%), 3 skin infections (8%), 2 urinary tract infections and one upper respiratory tract infection.
Microbiological characteristics of bacteria strains
Classification n (%)
Gram-positive bacteria: 17 (41%)
Staphylococcus sp: 9
S. epidermidis: 3 (methi-S: 1 - methi-R: 2)
- Gram positive Cocci: 16
S. aureus: 6 (methi-S: 4 - methi-R: 2)
Streptococcus sp: 4
- Streptococcus A : 2
- Streptococcus D bovis : 1
- Lactococcus lactis cremoris : 1
S. pneumoniae: 2
Enterococcus sp: 1
- Gram positive Bacilli: 1
Turicella otitidis: 1
Gram-negative bacteria: 24 (59%)
H. influenzae and parainfluenzae: 3
- Gram negative Bacilli: 23
- E. coli: 11
- Proteus mirabilis: 1
- Klebsiella oxytoca: 2
- Salmonella enterica: 1
- Citrobacter koseri: 1
Pseudomonas aeruginosa: 2
Sphingomonas paucimobilis: 1
Acinetobacter baumannii: 1
- Gram negative Cocci: 1
Moraxella catarrhalis: 1
Gram-negative bacilli predominated in both monomicrobial and polymicrobial infections. There were 13 Gram-negative monomicrobial infections and 11 Gram-positive monomicrobial infections. One diagnosis was made on the basis of serum antibodies against an intracellular bacterium, Chlamydia pneumoniae. In polymicrobial infections, combinations of several different Gram-negative bacteria were found in 4 cases. Combinations of several different Gram-positive species and combinations of Gram-negative and Gram-positive species were found in 2 cases each. However, we decided not to include four other doubtful combinations in which one of the bacteria was non-pathogenic and was not isolated from the same body site as the identified pathogenic species.
Multidrug-resistant bacteria (i.e. those resistant to two or more antibiotic families, including beta-lactams) were identified in 6 of the 41 infections (14.6%). Six of the 33 patients (18%) were infected with multidrug resistant bacteria. All S. pneumoniae were penicillin non-susceptible (n = 2) and almost half of all Staphylococcus sp. were methicillin-resistant (n = 4 out of 9). One of the two Pseudomonas aeruginosa isolates was ceftazidime-resistant and the only Sphingomonas paucimobilis isolate was found to be multidrug-resistant. There were no vancomycin-resistant Enterococcus isolates and no gentamycin-intermediate-susceptible S. aureus isolates. According to the antibiotic susceptibility profile, 24% of patients (n = 8) would have needed glycopeptides or cephaloridine.
Repartition of germ according to their tropism.
Uro-digestives germs: 19 strains
Enterococcus spp: 1
Acinetobacter baumannii : 1
Streptococcus bovis : 1
Cutaneous germs: 9 strains
Coagulase Negative Staphylococcus: 3
Staphylococcus aureus: 6
Respiratory germs: 8 strains
S. pneumoniae: 2
Haemophilus influenzae : 3
Pseudomonas aeruginosa: 2
Moraxella catarrhalis : 1
Other: 5 strains
Other Streptococcus: 3
Sphingomonas paucimobilis : 1
Turicella otitidis : 1
All three centers applied the same antibiotic treatment guidelines. The first-line treatment was either amoxicillin/clavulanic acid or a combination of cephalosporin and ciprofloxacin. The concordance between the guidelines and actual prescriptions was 65%; the discordances consisted of adjunction of an aminoglycoside in 41% of cases and use of a glycopeptide in 6% of cases. The outcome was favorable in most cases; the overall antibiotic therapy success rate was 82.8%. The first-line treatment was effective in 72.4% of cases (63 out of 87) but fell to 63.6% (n = 21 out of 33) in microbiologically documented episodes. Furthermore, the outcome of first-line treatment was in line with the in vitro susceptibility results in 84.8% of cases (28 out of 33). The median time interval between treatment initiation and apyrexia was 2 days (range: 1-12).
The mortality rate was 15% at day 7 and 33% at day 30, whereas the infection-related mortality rate was 16.1%. Ten of the 14 deceased patients (71%) had at least one severity criterion on admission.
Predictive factors of first-line antibiotic treatment failure.
Failure n = 24
Success n = 63
Mean age (years)
Age >60 years
WHO Score ≥2
No. of comorbidities>2
Small cell cancer
More than 1 chemotherapy
Already hospitalized patient
Altered general status
Gravity signs at admission
Neutropenia > 8 days
Neutrophils count <100/mm3
Haemoglobin <8 g/dl
Platelets < 20000/mm3
Decreasing of CRP
Undetermined origin of fever
Clinically documented fever (without microbiological documentation)
Microbiologically documented fever
Gram negative bacilli
Multidrug resistant bacteria in patient with at least one strain documented
Antibiotic treatment in last 6 month
Out patient first antibiotic treatment
Appropriateness between microbiology and antibiotherapy in patient with at least one strain documented
A multivariate analysis revealed that three factors were predictive of first-line antibiotic treatment failure: age>60 (p = 0.011), thrombocytopenia <20,000/mm3 (p < 0.01) and WHO score >2 (p = 0.012). The first two factors were also correlated with the overall failure of antibiotic treatment.
As expected, our population was typical of hospitalized lung cancer patients and primarily comprised elderly male smokers in poor general health (WHO score >2). The recent increase in the number of female smokers in France did not appear to have impacted on the present study. Likewise, the patients' prognoses were very poor, with a 33% overall mortality rate (half of which was due to febrile neutropenia) on day 30. In comparison with other studies, the mortality rate was high but may be explained by the fact that our study population was particularly severely affected: 60% had metastases and 31% were non-operable [14, 16].
Surprisingly, most of the identified bacteria were germs of the urinary and digestive tracts (rather than respiratory germs) and were predominantly detected by blood culture. In contrast, the clinically documented infections were mostly due to respiratory germs (53%, vs. 23% for digestive tract infections). These findings suggest that respiratory germs rarely cause bacteremia. Fifteen percent of the infections were multidrug-resistant and only 14% were hospital-acquired infections; these are low values for a population which is frequently hospitalized. However, multidrug-resistance and hospital-acquired infection did not appear to have a significant impact on the overall failure rate antibiotic treatment in our multivariate analysis, which identified only age >60 and thrombocytopenia < 20000/mm3 as factors. The presence of thrombocytopenia as a risk factor for treatment failure probably reflects the hemotoxicity of chemotherapy. As expected, a univariate analysis revealed that inappropriate antibiotic treatment was a risk factor for first-line treatment failure. However, the relatively low frequency of inappropriate treatment in our study (14% of all patients and 36.4% of the cases of microbiologically documented fever) did not prompt us to change our guidelines on antimicrobial treatment. Our guidelines did not include anti-pseudomonal penicillin or cabapenem contrary as Hughes et al. propose for high risk patients . However our patients were on the border between low and high risk patients. According to the same criteria, our patients had mostly those 4 following criteria for low risk patient: duration of neutropenia less than 7 days, resolution of neutropenia expected in <10 days, no neurological or mental changes, no appearance of illness (40%); in addition, according to the scoring index, most of our patients are in the 20-22 points range. In a very similar population, Rikimaru et al. observed that febrile neutropenia occurred more frequently in patients with poor performance status .
The present study had a number of limitations. Firstly, the data were non-exhaustive because of the exclusion of non-hospitalized patients. However, the proportion of cases with febrile neutropenia in our study (13%) is the same as that found by Cullen et al. in a prospective, UK study of patients with solid tumors (cancers and lymphoma). It may be that hospitalization of this type of patient is more frequent in France than in the UK. Secondly, bacteriological samples (especially urine and sputum samples) could have been contaminated and the pathogenic classification of any isolated germs was left to the attending physician in each case. Hence, there may have been confusion bias. Thirdly, this was a retrospective study, with all the weaknesses associated with this type of study design. However, in order to limit selection bias, we selected all consecutive hospitalized lung cancer patients and then excluded those lacking fever and neutropenia. Some patients may have been misclassified. Hence, all medical records charts were carefully reviewed by one of the authors (E.P.) according to pre-established criteria and were classified independently of the initially reported laboratory results.
The febrile neutropenia mortality rate observed here (16%) is greater than the value reported in a similar study (3%). This disparity may be due to the severity of our patients' disease.
As mentioned above, our results revealed a greater proportion of cases with fever of undetermined origin (46%) than those with microbiologically documented fever (38%) and clinically documented fever (16%). However, this difference has been found in other populations. Furthermore, fever of undetermined origin has a better prognosis [14, 18, 19].
Characteristics of infections in patients with lung cancer: review.
Markman et al.11
Small cell cancer neutropenic
S. aureus Bacillus spp
Matsui et al.12
All lung cancer
Leukocytes count <3000/mm3
Berghmans et al.20
All lung cancer neutropenic and non neutropenic
Niho et al.13
All lung cancer neutropenic
Lanoix et al.
All lung cancer neutropenic
Klastersky et al. reported on a huge cohort of 2142 patients with febrile neutropenia, of whom 164 had lung cancer . The aim of the latter study was to compare the severity of disease in bacteremic and non-bacteremic patients. The mortality rate was 8% in the small subgroup of lung cancer patients and the data were not analyzed further.
Kern reported that 20% of the infections were multidrug-resistant (versus 14.6% in the present study), with an antibiotic treatment failure rate of 22% (versus 18.2% here) . The mortality rate due to infection was 2.5% (versus 16% here) and the overall mortality rate at day 30 was 5% (versus 33% here). Of course, Kern's study population differed from ours and was considered to be at low risk of infection.
The present study emphasized two important points. Firstly, the microbiological profile of febrile neutropenia does not differ greatly from that seen in other types of neutropenia. Respiratory germs are less predominant than S. aureus and enterobacteriaceae and lung infection is not a prognostic factor (since only age and performance status were predictive of antibiotic treatment failure). Secondly, our results highlight the progression of the infection; the higher mortality rate was due more to the patients' fragility than the nature of the germs or the presence of multidrug resistance. Extremely broad-spectrum antibiotic therapy is not always necessary but treatment must take account of the patient's frailty and the presence of respiratory germs.
In order to circumvent the limitations of retrospective studies and avoid selection bias, it would be useful to perform the same type of study on a prospective basis and include non-hospitalized patients.
Acknowledgements and Funding
The authors would like to thank all the people who contributed to the project. The study did not receive any particular funding.
- Remontet L: Estimations nationales: tendances de l'incidence et de la mortalité par cancer en France entre 1978 et 2000. 190-193. BEH 41-42/2003
- Institut national de prévention et éducation pour la santé: Actualités Tabac. N° 2 juin/juillet. 1999, [http://www.inpes.sante.fr/ta/pdf/tabactu2.pdf]Google Scholar
- Aapro MS, Cameron DA, Pettengell R, Bohlius J, Crawford J, Ellis M, Kearney N, Lyman GH, Tjan-Heijnen VC, Walewski J, Weber DC, Zielinski C, European Organisation for Research and Treatment of Cancer (EORTC) Granulocyte Colony-Stimulating Factor (G-CSF) Guidelines Working Party: EORTC guidelines for the use of granulocyte-colony stimulating factor to reduce the incidence of chemotherapy-induced febrile neutropenia in adult patients with lymphomas and solid tumours. Eur J Cancer. 2006, 42 (15): 2433-53. 10.1016/j.ejca.2006.05.002.View ArticlePubMedGoogle Scholar
- Tjan-Heijnen VC, Postmus PE, Ardizzoni A, Manegold Ch, Burghouts J: Reduction of chemotherapy-induced febrile leucopenia by prophylactic use of ciprofloxacin and roxithromycin in small-cell lung cancer patients: An EORTC double-blind placebo controlled phase III study. Ann Oncol. 2001, 12: 1359-1368. 10.1023/A:1012545507920.View ArticlePubMedGoogle Scholar
- Cullen M, Steven MD, Billingham L, Gaunt C, Hastings M: Antibacterial prophylaxis after chemotherapy for solid tumors and lymphomas. N Engl J Med. 2005, 353: 988-998. 10.1056/NEJMoa050078.View ArticlePubMedGoogle Scholar
- Ray-Coquard I: Facteurs pronostics des neutropénies fébriles. Bulletin du Cancer. 2006, 93: 501-506.PubMedGoogle Scholar
- Hughes TW, Armstrong D, Bodey GP: 2002 Guidelines for the use of antimicrobial agents in neutropenic patients with cancer. Clin Infect Dis. 2002, 34: 730-751. 10.1086/339215.View ArticlePubMedGoogle Scholar
- Oppenheim BA: The changing pattern of infection in neutropenic patients. J Antimicrob Chemother. 1998, 41: 7-11. 10.1093/jac/41.suppl_4.7.View ArticlePubMedGoogle Scholar
- Poyart C, Morand P, Buzyn A: Étiologie des infections bactériennes chez les patients neutropéniques fébriles: rôle du laboratoire dans le diagnostic. Presse Med. 2004, 33: 460-466. 10.1016/S0755-4982(04)98633-2.View ArticlePubMedGoogle Scholar
- Cordonnier C, Buzyn A, Leverger G: Epidemiology and risk factors for gram-positive coccal infections in neutropenia: toward a more targeted antibiotic strategy. Clin Infect Dis. 2003, 36: 149-158. 10.1086/345435.View ArticlePubMedGoogle Scholar
- Markman M, Abeloff MD: Management of hematologic and infectious complications of intensive induction therapy for small cell carcinoma of the lung. Am J Med. 1983, 74: 741-746. 10.1016/0002-9343(83)91061-6.View ArticlePubMedGoogle Scholar
- Matsui K, Masuda N, Takada M, Kusunoki Y, Fukuoka M: A randomized trial comparing imipenem/cilastatine alone with latamoxef plus tobramycin in febrile neutropenic patients with lung cancer. Japan J Clin Oncol. 1991, 21: 428-434.Google Scholar
- Niho S, Ohe Y, Goto K: Randomized trial of oral versus intravenous antibiotics in low-risk febrile neutropenic patients with lung cancer. Japan J Clin Oncol. 2004, 34: 69-73. 10.1093/jjco/hyh019.View ArticleGoogle Scholar
- Kern WV, Cometta A, De Bock R, Langenaeken J, Paesmans M, Gaya H: Oral versus intravenous empirical antimicrobial therapy for fever in patients with granulocytopenia who are receiving cancer chemotherapy. N Engl J Med. 1999, 341: 312-318. 10.1056/NEJM199907293410502.View ArticlePubMedGoogle Scholar
- Klastersky J, Paesmans M, Rubenstein EB, Boyer M, Elting L, Feld R, Gallagher J, Herrstedt J, Rapoport B, Rolston K, Talcott J: The Multinational Association for Supportive Care in Cancer risk index: A multinational scoring system for identifying low-risk febrile neutropenic cancer patients. J Clin Oncol. 2000, 18: 3038-51.PubMedGoogle Scholar
- Klastersky J, Ameye L, Maertens J, Georgala A, Muanza F, Aoun M, Ferrant A, Rapoport B, Rolston K, Paesmans M: Bacteraemia in febrile neutropenic cancer patients. Int J Antimicrob Agents. 2007, 30S: S51-9.View ArticleGoogle Scholar
- Rikimaru T, Ichiki M, Ookubo Y, Matumoto K, Mimori Y: Pronostic significance of febrile episodes in lung cancer patients receiving chemotherapy. Support Care Cancer. 1998, 6: 396-401. 10.1007/s005200050183.View ArticlePubMedGoogle Scholar
- Cometta A, Zinner S, de Bock R, Calandra T, Gaya H: Piperacillin-tazobactam plus amikacin versus ceftazidime plus amikacin as empiric therapy for fever in granulocytopenic patients with cancer. Antimicrob Agents Chemother. 1995, 39: 445-452.View ArticlePubMedPubMed CentralGoogle Scholar
- Cometta A, Calandra T, Gaya H, Zinner SH, de Bock R: Monotherapy with meropenem versus combination therapy with ceftazidime plus amikacin as empiric therapy for fever in granulocytopenic patients with cancer. Antimicrob Agents Chemother. 1996, 40: 1108-1115.PubMedPubMed CentralGoogle Scholar
- Berghmans T, Sculier JP, Klastersky J: A prospective study of infections in lung cancer patients admitted to the hospital. Chest. 2003, 124: 114-120. 10.1378/chest.124.1.114.View ArticlePubMedGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/11/183/prepub
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