Contingency planning for a deliberate release of smallpox in Great Britain - the role of geographical scale and contact structure
© House et al; licensee BioMed Central Ltd. 2010
Received: 30 July 2009
Accepted: 14 February 2010
Published: 14 February 2010
In the event of a release of a pathogen such as smallpox, which is human-to-human transmissible and has high associated mortality, a key question is how best to deploy containment and control strategies. Given the general uncertainty surrounding this issue, mathematical modelling has played an important role in informing the likely optimal response, in particular defining the conditions under which mass-vaccination would be appropriate. In this paper, we consider two key questions currently unanswered in the literature: firstly, what is the optimal spatial scale for intervention; and secondly, how sensitive are results to the modelling assumptions made about the pattern of human contacts?
Here we develop a novel mathematical model for smallpox that incorporates both information on individual contact structure (which is important if the effects of contact tracing are to be captured accurately) and large-scale patterns of movement across a range of spatial scales in Great Britain.
Analysis of this model confirms previous work suggesting that a locally targeted 'ring' vaccination strategy is optimal, and that this conclusion is actually quite robust for different socio-demographic and epidemiological assumptions.
Our method allows for intuitive understanding of the reasons why national mass vaccination is typically predicted to be suboptimal. As such, we present a general framework for fast calculation of expected outcomes during the attempted control of diverse emerging infections; this is particularly important given that parameters would need to be interactively estimated and modelled in any release scenario.
Stopping transmission of the smallpox (variola) virus amongst the human population was one of the greatest public health triumphs of the twentieth century; and yet since the events of 11 September 2001 the possibility of its reemergence has been under increased study . Effective contingency planning for such a scenario requires knowledge of the optimal deployment and use of case isolation, contact tracing and vaccination. Since a series of controlled experiments to inform such decisions is not possible, we are forced to rely on mathematical modelling to improve the evidence base for emergency preparedness and response. Recent simulation models [2–5] typically conclude that, provided a given smallpox outbreak can be well controlled, the emphasis should be on case isolation, contact tracing and targeted vaccination rather than immediate country-level mass vaccination, due to the frequency of adverse effects from vaccine and the likely high efficacy of other measures. Targeting of vaccination can either be exclusively towards suspected cases found by contact tracing, or can also involve a policy of local 'ring vaccination' of the population in the vicinity known cases. Evidence exists that such ring vaccination may provide additional benefit over individually targeted measures . However, two questions remain and form the main considerations of this paper: the exact conditions for which ring vaccination is optimal, and the scale at which such vaccination should take place--in particular we focus on the interaction between ring vaccination and contact tracing in a clustered network of contacts.
When considering optimal ring vaccination in any epidemiological context, the key issue is the spatial scale for intervention. This presents a technical challenge, since spatial models of disease transmission are typically much more complex than non-spatial models. Local vaccination is also not applied in isolation, but is deployed together with other control measures that must be captured by the model. One of the most widely used additional measures is contact tracing, which seeks to identify infected individuals before they become fully symptomatic by tracing the contacts (and therefore potential secondary cases) from each identified case. Theoretical work shows that failure to account for the underlying structure of the contact network when considering contact tracing can cause severe, qualitative errors in model results . We therefore find that our model choice is constrained by the epidemiology, dynamics, and types of control measure that we wish to capture.
Three main model types have been used to investigate the spread and control of smallpox . Mean-field models [, §8.3] have the advantage of being a relatively tractable set of differential equations, but suffer from the underlying assumption that transmission occurs at random within the entire population. Metapopulation models  are based on the observation that most infectious individuals create the majority of their secondary cases within their local environment; in these metapopulation models the population is therefore aggregated in local administration units with most transmission occurring within each unit and at random. Individual-based simulation models [2–4] are generally considered the most realistic and aim to capture the full contact structure between all individuals in the population, often assigning individuals to households, schools and workplaces where there is known to be a higher risk of transmission. Here we define a model that includes mixtures from all three of the above methods: it is based on differential equation and therefore is rapid to simulate allowing rigorous sensitivity analysis; it retains the metapopulation concept of local and longer-range transmission so can be used to consider regional (ring) vaccination; and it incorporates elements of local contact structure and can therefore reliably capture the effects of contact tracing.
Individual-based simulation can in principle incorporate any population structure and interventions necessary, and as such there will always be an important role in contingency planning for computationally intensive models that aim for maximum realism. Nevertheless, there are limitations to these approaches that motivate our methodology of developing a new, more parsimonious model. Most important for our purposes are the problems of parameterisation, numerical tractability and transparency. With such individual-based simulations there is always the temptation to include ever more complexity, however any model is necessarily a caricature of reality and so the quest for ever greater realism can never be fulfilled. While computers and algorithms continue to improve, increasing the number of individuals that can be directly simulated and decreasing the time to perform a simulation, considering the whole population of England, Wales and Scotland still involves significant computational resources. While baseline results can be obtained at these population sizes reasonably quickly, applications that require large number of model realisations such as comprehensive sensitivity analysis, real-time parameter fitting and determination of optimal strategies quickly become highly time-consuming. The development of models and methods to implement such applications remains necessary, however complementary approaches that are more abstract can significantly reduce the computational burden.
A model that incorporates synthetic data on individuals and their contacts almost by definition involves many more parameters than can be measured directly, and as such assumptions have to be made on the basis of available data. For example, if an explicit network is generated as in , then there is really a network parameter for every pair of individuals in the population modelled. Given the gap between available data on human contacts and the information contained in an explicit network, a complementary approach to individual-based simulation is to develop mathematical approaches that give epidemiological outputs based on statistical properties of contact networks. Such network properties can often be measured directly, and the uncertainty in them estimated systematically. One published methodology for this, applied to smallpox, is to use a branching-process model . Such an approach does not, however, allow the consideration of clustering--the possibility that an individual's contacts also contact each other. Work not directly related to smallpox, based on pairwise models, has shown that clustering has a major impact on transmission and tracing dynamics [6, 11, 12]. This leads us to extend pairwise models to enable smallpox to be modelled whilst considering clustering. A thorough treatment of this methodology is available in [, Chapter 7] and .
Model parameters, together with baseline value and range if varied during analysis.
Rate of transition from latent to prodromal
Rate of transition from prodromal to infectious
Rate of transition from infectious to removed
Case fatality risk
Probability of case isolation success
Rate of transition from isolated to removed
Probability of contact tracing success
Vaccine efficacy when susceptible
Vaccine efficacy when latent
Rate of transition from observed to removed or vaccinated
Proportion of population contraindicated for mass vaccination
Number of index cases
Number of index cases for secondary outbreak
Number of clinical cases prior to detection
Number of clinical cases prior to detection for secondary outbreak
Vaccine fatality risk
10-5(0 -- 10-5)
Basic reproductive ratio
5 (3 -- 7)
Prodromal type reproductive ratio
0.5 (0.1 -- 1.5)
Movement reductions when infectious
0.9 (0 -- 1)
Rate of mass vaccination
N/7 (see Figure 3(c))
Population size of region
105(see Figure 3(a))
0.4 (see Figure 3(d))
Number of regions of same type as outbreak region
n/a (see Figure 3(a))
Number of regions at relevant scale initially infected
1 (1 -- 10)
Neighbourhood size (contacts per individual)
17 (5 -- 50)
0 (0 -- 0.5)
Smallpox natural history
When we come to consider transmission, the most important parameter is the basic reproductive ratio, R 0, which represents the expected number of secondary cases produced by a typical primary case early in the epidemic. Given that both prodromal and fully infectious individuals can transmit infection, we can split R 0 = R P + R I where R P is the expected number of secondary cases caused during the prodromal stage and R I the number during the fully infectious stage. Our default assumptions are that R 0 = 5, R P = 0.1 × R 0, R I = 0.9 × R 0 [14, 15, 17].
Contact network structure
The full network of human contacts capable of spreading infectious disease is undoubtedly complex, highly structured and dynamic. At the same time, there are many network statistics that are known to be epidemiologically important, however these are all based on the facts that transmission-relevant contacts are finite and non-random (captured by the mean number of contacts per individual, n) and that contacts are often shared by individuals (captured by the clustering coefficient ϕ). These two parameters are, therefore, an appropriate choice for our purposes and we vary them significantly during our analysis. We note that in many cases the heterogeneity in the number of contacts per individual can play an important role; but for the early stages of an outbreak considered here the effects of this heterogeneity can largely be subsumed into the parameterisation of R 0.
The mean number of contacts, n, has been estimated and we take it to be 17 people as a default [18, 19]. The clustering coefficient ϕ, which is equal to the mean proportion of shared contacts between two linked individuals, has yet to be measured in an accurate way, and so our baseline value is ϕ = 0 for comparison with existing unclustered models. Since we know that there are many internally well-connected groups and cliques within human populations, such as households, schools and workplaces, it is most likely that ϕ is far from 0 and so large values are considered when we generalise.
So prodromal individuals infect susceptible individuals that they are connected to at a rate τ P and infectious individuals infect susceptible individuals that they are connected to at a rate τ I .
If we were not considering a model with an explicit contact network, then the relationship between underlying transmission rates and observables can be dealt with in a rigorous way, such as in . On a network, however, the relationship between transmission rates and the reproduction numbers R 0, R P , R I discussed above is subtle, and the technical details of our approach are given in Additional file 1. The important conclusion from this technical development is that there are really two types of reproduction number that one should consider. The first, which we write r 0 (composed of r I and r P ), relates to population-level measurements of incidence and prevalence and their early growth rate, while the second, which we write ℛ0 (and is composed of ℛ I and ℛ P ) relates to individual-level measurements made during contact tracing and is more true to the verbal definition. We consider both types of reproduction number in our analysis.
We now consider methods and parameters for the various interventions that are typically considered in smallpox contingency planning.
The first step in outbreak containment is to isolate symptomatic cases as soon as possible, to stop transmission of disease. We model this by taking a proportion θ = 0.9 of the prodromal cases into a new quarantined class, Q, rather than the infectious I class at the end of the prodromal period . Isolated cases are released into the recovered R class with rate constant g Q = (1/20) days-1 when they are assumed to pose no further risk of transmission . This measure on its own therefore reduces the number of secondary cases produced to R P + 0.1 × R I = 1 under our baseline assumptions, which is consistent with existing work and expert opinion .
Contact tracing of known (and hence isolated) cases takes place through attempts to find the individuals potentially infected through epidemiologically relevant contacts. Some individuals found in this way will still be susceptible, some will be latent cases, and others will have developed symptoms. We assume that traced individuals displaying both rash and less specific prodromal fever are isolated an enter the quarantined Q class. Traced contacts not displaying any symptoms will be subject to the same observation protocols until they either develop symptoms or conclusively pass the upper limits of the incubation period. We distinguish in the model between susceptible individuals under observation, O, and traced cases still in the latent stage, E T .
For a given isolated individual who is subject to contact tracing, we assume that each contact in the transmission network is discovered independently with a probability of ∈ = ρ/(ρ + g Q ) = 0.8 . The mathematical reasoning for this is given in Additional file 1.
The rate g O = (1/15) days-1 is given by the length of the observation period for observed suspected cases .
where v is the absolute rate (people per day) at which a population can be vaccinated. The estimation of this quantity is really a question for operational research, and therefore falls outside the scope of this paper. From discussions with the Department of Health, we were able to obtain their estimate that the country could be vaccinated within a week. Since our results are not particularly sensitive to this parameter, the implications of varying it are reasonably clear (slower vaccination is less effective and vice versa), and we do not have access to sufficient information to make an estimate of our own, we make use of the value of one week. v is therefore equal to the population of Great Britain not contraindicated for vaccination (70% of 57 million) multiplied by the inverse time required to vaccinate, 1/7 days-1 .
One of the main aims of our work is to consider the optimal spatial scale for intervention, and so here we present the data and modelling framework within which such assessments can be made.
The NUTS classification
As in the section above on vaccination, the Department of Health provided us with an estimate of the time to vaccinate a district as three days. We then extrapolate to Figure 3(c) in order to consider multiple spatial scales, although again our results are not highly sensitive to these parameters.
Interaction between regions
Our approach to spatial interaction between regions considers disease escape from the region around the initial cases through a tractable model known as a Poisson process. Such an approach has also been suggested as a method for model simplification for pandemic influenza . The mathematical description of this process is presented in Additional file 1.
In order to make use of this method, we need to have a measure of the propensity of a region's resident population to leave the area (and hence transfer infection) on a day to day basis. To our knowledge, there is no technical term for this value; we use the term outwardness and denote it ξ. We then estimate the outwardness of a geographical scale to be the median proportion of workers living in a region at that scale and working outside it (i.e. we assume that non-workers are, in this respect, like workers). We then extrapolate the raw data in Figure 3(b) through linear interpolation on a logarithmic population scale to get the general relationship between population and outwardness shown in Figure 3(d).
Here, we parameterise the significantly reduced movement of symptomatic infectious individuals through multiplying their probability of travel by a factor (1 - κ), with the default value of κ = 0.9 .
Optimisation of response
Our results consider a six-month outbreak, centred on either a single region or number of regions. For each region, we are able to calculate an expected mortality from the final numbers vaccinated and removed, and the respective mortality risks δ V and δ. We are also able to calculate a probability of escape from the region. Logistical and ethical factors become important in the consideration of which outcomes are most desirable, however, and so our approach is to consider a simply described optimal outcome within two release scenarios.
Initial cases and detection threshold
Our first assumption concerning initial cases is that they are concentrated, and the epidemic starts with E 0 = 10 latent cases in one area. While in principle this could be significantly higher, the more severe epidemic arising from such a situation may favour mass vaccination as an intervention. We therefore choose to consider an initial outbreak size where there is the strong possibility of containment through case isolation and contact tracing alone.
We assume that until a certain number of fully symptomatic cases has been seen and diagnosed, no public response is instigated. The triggering of interventions is modelled by introducing control measures to the system once [I] + [R] ≥ I trig. We take I trig= 4, for the same reason as our relatively conservative choice of E 0 above.
In the event that secondary areas are infected, we assume that in a situation of heightened awareness and public health response, the secondary epidemic is initialised by = 1 initial cases and detected once = 1 symptomatic cases are present.
An alternative scenario is to consider M 0 = 10 maximally dispersed initial cases, each in a distinct region of the size under consideration. In this case, we let the initial regions take 'secondary' parameters; since the outbreaks are simultaneous, this means that the expected national prevalence of clinical infection upon triggering of intervention will be M 0. This is reasonable since dispersed cases are collectively less likely to trigger intervention.
In terms of interpretation, our baseline assumption for regions of relative small sizes would correspond to an accidental/intentional release from a terror cell operating from a domestic address, and at larger sizes a release involving the resident population of a city or region. The dispersed assumption would, on the other hand, involve the other extreme of a release involving a largely transient population of commuters or shoppers. We note that at country level (Great Britain) the dispersed scenario should be interpreted as identical to the NUTS1 level dispersed scenario.
Minimisation of expected mortality
The decision about what constitutes an optimal outcome is essentially political, and so our focus is on how epidemiological outputs vary with spatial scale and contact network structure. Nevertheless, we also wish to demonstrate how optimisation can be considered and so also calculate the total expected mortality in primary and secondary affected regions as a simply described and calculated quantity for optimisation. The full details of calculation of this quantity are in Additional file 1.
Results and Discussion
Using the geographical framework discussed above, varying the vaccine mortality risk and mobility of fully symptomatic cases, but otherwise using the baseline parameters of Table 1, gives the results shown in Figure 4(c,d).
We find, as shown in Figure 4(c), that below a particular population size close to two million (if vaccine-induced mortality δ V is 10 deaths per million vaccinated) local mass vaccination is optimal, with the preferred spatial scale being defined such that there is reasonable confidence that all existing cases are contained at that scale. Above this critical population size, the number of vaccine-induced deaths becomes unacceptably large. Our conclusion is that, even if vaccine-induced mortality is significantly lower, for the small release considered here vaccination of the whole country is likely to be unnecessary given that vaccination at smaller spatial scales and more individually targeted approaches will have brought the epidemic under control.
In terms of escape of infection, Figure 4(d) shows that both the reduced mobility of symptomatic cases and local mass vaccination have a significant impact on final probability. While this is not plotted for clarity, increasing κ still further does not have a large effect. The dominant trend is, in any case, that the outwardness of a region is the main determining factor of magnitude of escape probability.
When examining sensitivity to parameter choices, it is important to consider which basic observables of the epidemic should be maintained while the parameters are varied. Here, we have decided to fix the basic reproductive ratio as this is undoubtedly the key observation from any epidemic. For example, as we vary the number of contacts, the transmission rate per contact is varied to compensate for this change. This is analogous to ensuring that all models are fit to the same early epidemic behaviour. One difficulty with fitting to the basic reproductive ratio is that it can be measured in two main ways: either directly by examining the contacts of each infected case, or indirectly by calculating the early growth rate of the epidemic. Unfortunately, for epidemics with complex natural histories and network-based transmission these two methods of calculating the basic reproductive ratio are not in direct agreement, and we therefore show two sets of results in which either measurement is held constant. To make this distinction clear, we denote an individually measured reproduction number ℛ0 and one inferred from early growth r 0.
We have found relatively small effects from varying the neighbourhood size n at constant R 0 (Figure 5(a)), although varying the reproductive ratio itself has the strong but predictable effect of favouring mass vaccination for larger values of R 0 (Figure 5(b)). What we find significantly more important is the amount of transmission during the prodromal phase of disease, parameterised by R P (Figure 5(c)). While this is highly unlikely to be over 1, a similar effect would be seen from a delay in isolation of infectious individuals, which can be shown in a general manner using threshold arguments . Investigating the effects of clustering (Figure 5(d)) gives strong results, which are both subtle and counter-intuitive, although some theoretical progress has been made in understanding the impact of clustering on contact tracing . In particular, we see in Figure 5(d) that whether R 0 has been estimated from early growth in prevalence or from contact-tracing data has a profound effect on the impact of clustering.
Optimisation and global sensitivity
Parameters not varied
There are a large number of tasks for realistic, informative smallpox modelling, and our particular focus has been on outbreak severity, the impact of contact-network structure, spatial scale and level of undetected transmission. It is worth considering briefly the likely impact of two other extensions to simple models known to be important for general policy conclusions.
Firstly, we have assumed simple rates of transmission between infectious states rather than a more sophisticated class-age structure. Our methodology allows for the inclusion of extra realism of this form through the method of stages, where clinically distinct disease states are broken down into additional sub-compartments. This brings significant extra computational overheads, however when considering a binary choice about vaccination the current understanding of such realism is that it should not qualitatively modify our results, and would be most important if our approach were extended to an accurate system of real-time estimation.
Secondly, we have assumed homogeneity at the individual level, while risks of death, mixing patterns and spatial location are likely to be highly heterogeneous. Again, given that we were only considering mean behaviour of the system this does not invalidate our approach, but would be important if policy decisions needed to be calibrated to 'reasonable worst case' rather than expected outcomes.
In dealing with the optimisation of public-health response to deliberate release of smallpox, we are considering a highly complicated system that is not directly amenable to experimental testing. This means that there will always be a degree of uncertainty associated with conclusions presented, however strongly these manifest themselves in a model.
Despite this general caveat, investigation of the problem for Great Britain suggests that, for a wide variety of parameter choices, and with differing modelling approaches, a vaccination strategy that involves a wider section of the population than the traced contacts of isolated cases but one that stops short of vaccinating the whole country is likely to be optimal. Such a conclusion is, we believe, likely to be robust in the event of a relatively small initial outbreak and given our best estimates of the contagiousness and natural history of variola. It could be overturned for a particularly virulent or large outbreak, however similar measures were taken during the eradication programme and were effective in controlling historical outbreaks . Additionally, a larger outbreak may be observed sooner, leading to earlier detection and isolation of cases. Another key message is that delays in case isolation, or equivalently more transmission in the prodromal phase than was previously observed, are extremely significant, and that slow response has the potential to present the most significant barrier to the efficacy of non-pharmaceutical interventions. Conversely, there are significant advantages to faster case isolation and treatment.
We have generalised on previous work in two main ways. Firstly, we have extended the treatment of contact networks to include the realistic assumption of clustering, which has an extremely important impact on the efficacy of contact tracing. However, the measurement of even basic quantities like the clustering coefficient ϕ for epidemiologically relevant contacts has yet to be attempted at a large geographical scale, particularly in non-household contexts, and we suggest that this could be an important measurement to make given its substantial effect on predicted outcomes.
Secondly, we have considered human movement patterns across a full range of geographical scales, enabling the calculation of an optimal scale for intervention. Furthermore, the techniques in this paper can be used to analyse situations where the numbers people leaving an area are inferable but the full region to region 'commuting' behaviour is unknown. As such this could be applied to other countries that don't collect detailed workplace location information in their census programmes.
There are many further generalisations of our work that could be considered, which would be of use regarding a response to a wide variety of emerging infectious agents. In particular, the inclusion of higher-order clusters in human contact networks, such as households, workplaces and social groups, is likely to be of significant importance. Also likely to be significant is the modification of 'baseline' patterns of movement and social interaction in response to perceived risk of infection. Consideration should also be made of the logistical constraints on local- and national-scale policies. We hope that the current work presents a useful foundation for the consideration of these and other questions.
This work was funded by the Department of Health for England and the Home Office Counter Terrorism and Intelligence Directorate through the Health Protection Agency, and also by the UK Medical Research Council (Grant Number G0701256). We would like to thank Joseph Egan, Steve Leach, Judith Legrand and Neil Ferguson for helpful discussions in project meetings during the preparation of these results.
- Lane HC, Montagne JL, Fauci AS: Bioterrorism: A clear and present danger (vol 7, pg 2001). Nature Medicine. 1271, 8: 87-87.Google Scholar
- Riley S, Ferguson NM: Smallpox transmission and control: Spatial dynamics in Great Britain. Proceedings of the National Academy of Sciences of the United States of America. 2006, 103 (33): 12637-12642. 10.1073/pnas.0510873103.View ArticlePubMedPubMed CentralGoogle Scholar
- Longini IM, Halloran ME, Nizam A, Yang Y, Xu S, Burke DS, Cummings DAT, Epstein JM: Containing a large bioterrorist smallpox attack: a computer simulation approach. Int J Infect Dis. 2007, 11 (2): 98-108. 10.1016/j.ijid.2006.03.002.View ArticlePubMedGoogle Scholar
- Burke DS, Epstein JM, Cummings DAT, Parker JI, Cline KC, Singa RM, Chakravarty S: Individual-based computational modeling of smallpox epidemic control strategies. Acad Emerg Med. 2006, 13 (11): 1142-1149. 10.1111/j.1553-2712.2006.tb01638.x.View ArticlePubMedGoogle Scholar
- Hall IM, Egan JR, Barrass I, Gani R, Leach S: Comparison of smallpox outbreak control strategies using a spatial metapopulation model. Epidemiol Infect. 2007, 135 (07): 12-10.1017/S0950268806007783.View ArticleGoogle Scholar
- House T, Keeling MJ: The impact of contact tracing in clustered populations. PLoS Computational Biology. 2010, Under reviewGoogle Scholar
- Ferguson NM, Keeling MJ, Edmunds WJ, Gani R, Grenfell BT, Anderson RM, Leach S: Planning for smallpox outbreaks. Nature. 2003, 425: 681-685. 10.1038/nature02007.View ArticlePubMedGoogle Scholar
- Keeling MJ, Rohani P: Modeling Infectious Diseases in Humans and Animals. 2007, Princeton University PressGoogle Scholar
- Porco TC, Holbrook KA, Fernyak SE, Portnoy DL, Reiter R, Aragdón TJ: Logistics of community smallpox control through contact tracing and ring vaccination: a stochastic network model. BMC public health. 2004, 4: 34-10.1186/1471-2458-4-34.View ArticlePubMedPubMed CentralGoogle Scholar
- Kretzschmar M, Hof van den S, Wallinga J, van Wijngaarden J: Ring vaccination and smallpox control. Emerging Infectious Diseases. 2004, 10 (5): 832-41.View ArticlePubMedPubMed CentralGoogle Scholar
- Keeling MJ: The effects of local spatial structure on epidemiological invasions. Proc R Soc Lond B. 1999, 266: 859-867. 10.1098/rspb.1999.0716.View ArticleGoogle Scholar
- Eames K, Keeling M: Contact tracing and disease control. P Roy Soc Lond B. 2003, 270: 2565-2571. 10.1098/rspb.2003.2554.View ArticleGoogle Scholar
- Keeling MJ, Eames KTD: Networks and epidemic models. Journal of The Royal Society Interface. 2005, 2 (4): 295-307. 10.1098/rsif.2005.0051.View ArticlePubMed CentralGoogle Scholar
- Mack TM: Smallpox in Europe, 1950-1971. Journal of Infectious Diseases. 1972, 125 (2): 161-169.View ArticlePubMedGoogle Scholar
- Eichner M, Dietz K: Transmission potential of smallpox: estimates based on detailed data from an outbreak. American Journal of Epidemiology. 2003, 158: 110-117. 10.1093/aje/kwg103.View ArticlePubMedGoogle Scholar
- Koplan J, Azizullah M, Foster S: Urban hospital and rural village smallpox in Bangladesh. Tropical Geographical Medicine. 1978, 30 (3): 355-358.PubMedGoogle Scholar
- Fraser C, Riley S, Anderson RM, Ferguson NM: Factors that make an infectious disease outbreak controllable. Proceedings of the National Academy of Sciences of the United States of America. 2003, 101 (16): 6146-6151. 10.1073/pnas.0307506101.View ArticleGoogle Scholar
- Edmunds W, O'Callaghan C, Nokes D: Who mixes with whom? A method to determine the contact patterns of adults that may lead to the spread of airborne infections. P Roy Soc Lond B. 1997, 264 (1384): 949-957. 10.1098/rspb.1997.0131.View ArticleGoogle Scholar
- Mossong J, Hens N, Jit M, Beutels P, Auranen K, Mikolajczyk R, Massari M, Salmaso S, Tomba GS, Wallinga J, Heijne J, Sadkowska-Todys M, Rosinska M, Edmunds WJ: Social contacts and mixing patterns relevant to the spread of infectious diseases. PLoS Med. 2008, 5 (3): 381-391. 10.1371/journal.pmed.0050074.View ArticleGoogle Scholar
- Inaba H, Nishiura H: The state-reproduction number for a multistate class age structured epidemic system and its application to the asymptomatic transmission model. Mathematical Biosciences. 2008, 216: 77-89. 10.1016/j.mbs.2008.08.005.View ArticlePubMedGoogle Scholar
- Gani R, Leach S: Transmission potential of smallpox in contemporary populations. Nature. 2001, 414: 748-751. 10.1038/414748a.View ArticlePubMedGoogle Scholar
- Nishiura H, Danon L, Egan J, Eichner M, Ferguson N, Flahault A, Hall I, House T, Keeling MJ, Kretzschmar M, Leach S: Containing a smallpox outbreak following deliberate release. 2010.Google Scholar
- Fenner F, Henderson DA, Arita I, Jezek Z, Ladnyi ID: Smallpox and its Eradication. 1988, Geneva, Switzerland: World Health OrganizationGoogle Scholar
- Kretzschmar M, Wallinga J, Teunis P, Xing S, Mikolajczyk R: Frequency of adverse events after vaccination with different vaccinia strains. PLoS Med. 2006, 3 (8): e272-10.1371/journal.pmed.0030272.View ArticlePubMedPubMed CentralGoogle Scholar
- Kemper AR, Davis MM, Freed GL: Expected adverse events in a mass smallpox vaccination campaign. Effective Clinical Practice. 2002, 5 (2): 98-99.Google Scholar
- Office for National Statistics: Nomenclature of Units for Territorial Statistics (NUTS)/Local Administrative Units (LAUs). 2009, [http://www.statistics.gov.uk/geography/nuts.asp]Google Scholar
- Office for National Statistics : 2001 Census: Special Workplace Statistics (England, Wales and Northern Ireland) [computer file]. 2003, [ESRC/JISC Census Programme, Centre for Interaction Data Estimation and Research (University of Leeds). Census output is Crown copyright and is reproduced with the permission of the Controller of HMSO and the Queen's Printer for Scotland.].Google Scholar
- Office for National Statistics: 2001 Census: Standard Area Statistics (England and Wales) [computer file]. 2003, [ESRC/JISC Census Programme, Census Dissemination Unit, Mimas (University of Manchester). Census output is Crown copyright and is reproduced with the permission of the Controller of HMSO and the Queen's Printer for Scotland.].Google Scholar
- General Register Office for Scotland: 2001 Census: Standard Area Statistics (Scotland) [computer file]. 2003, [ESRC/JISC Census Programme, Census Dissemination Unit, Mimas (University of Manchester). Census output is Crown copyright and is reproduced with the permission of the Controller of HMSO and the Queen's Printer for Scotland.].Google Scholar
- General Register Office for Scotland: 2001 Census: Special Travel Statistics (Scotland) [computer file]. 2003, [ESRC/JISC Census Programme, Centre for Interaction Data Estimation and Research (University of Leeds). Census output is Crown copyright and is reproduced with the permission of the Controller of HMSO and the Queen's Printer for Scotland.].Google Scholar
- Tomba GS, Wallinga J: A simple explanation for the low impact of border control as a countermeasure to the spread of an infectious disease. Mathematical Biosciences. 2008, 214: 70-72. 10.1016/j.mbs.2008.02.009.View ArticleGoogle Scholar
- Kerrod E, Geddes AM, Regan M, Leach S: Surveillance and control measures during smallpox outbreaks. Emerging Infectious Diseases. 2005, 11 (2): 291-297.View ArticlePubMedPubMed CentralGoogle Scholar
- Nishiura H, Brockman SO, Eichner M: Extracting key information from historical data to quantify the transmission dynamics of smallpox. Theoretical Biology and Medical Modelling. 2008, 5: 1-12. 10.1186/1742-4682-5-20.View ArticleGoogle Scholar
- Department of Health: Smallpox mass vaccination: An operational planning framework. 2005, [http://www.dh.gov.uk/en/Publicationsandstatistics/Publications/PublicationsPolicyAndGuidance/DH_4114017]Google Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2334/10/25/prepub
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.